PMID- 16980965
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20161019
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 9
IP  - 10
DP  - 2006 Oct
TI  - An essential role for beta-actin mRNA localization and translation in 
      Ca2+-dependent growth cone guidance.
PG  - 1265-73
AB  - Axon pathfinding requires directional responses of growth cones to extracellular 
      cues, which have been shown to involve local synthesis of protein. The identity 
      and functions of the locally produced proteins remain, however, unclear. Here we 
      report that Ca(2+)-dependent bidirectional turning of Xenopus laevis growth cones 
      requires localized distribution and translation of beta-actin messenger RNA. Both 
      beta-actin mRNA and its zipcode-binding protein, ZBP1, are localized at the 
      growth cone and become asymmetrically distributed upon local exposure to 
      brain-derived neurotrophic factor (BDNF). Inhibition of protein synthesis or 
      antisense interference with beta-actin mRNA-ZBP1 binding abolishes both 
      Ca(2+)-mediated attraction and repulsion. In addition, attraction involves a 
      local increase in beta-actin, whereas repulsion is accompanied by a local 
      decrease in beta-actin; thus, both produce a synthesis- and ZBP1 
      binding-dependent beta-actin asymmetry but with opposite polarities. Together 
      with a similar asymmetry in Src activity during bidirectional responses, our 
      findings indicate that Ca(2+)-dependent spatial regulation of beta-actin 
      synthesis through Src contributes to the directional motility of growth cones 
      during guidance.
FAU - Yao, Jiaqi
AU  - Yao J
AD  - Department of Neuroscience and Cell Biology, University of Medicine and Dentistry 
      of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New 
      Jersey 08854, USA.
FAU - Sasaki, Yukio
AU  - Sasaki Y
FAU - Wen, Zhexing
AU  - Wen Z
FAU - Bassell, Gary J
AU  - Bassell GJ
FAU - Zheng, James Q
AU  - Zheng JQ
LA  - eng
GR  - R29 NS036241/NS/NINDS NIH HHS/United States
GR  - HD46368/HD/NICHD NIH HHS/United States
GR  - NS36241/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060917
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Nat Neurosci. 2006 Oct;9(10):1201-3. PMID: 17001333
MH  - Actins/*genetics
MH  - Animals
MH  - Blotting, Western/methods
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Drug Interactions
MH  - Embryo, Nonmammalian
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Antibody Technique/methods
MH  - Glycoproteins/genetics/metabolism
MH  - Growth Cones/drug effects/*physiology
MH  - In Situ Hybridization/methods
MH  - Neurons/*cytology/physiology
MH  - Protein Biosynthesis/drug effects/*physiology
MH  - RNA, Messenger/*physiology
MH  - Time Factors
MH  - Xenopus laevis
EDAT- 2006/09/19 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/09/19 09:00
PHST- 2006/07/18 00:00 [received]
PHST- 2006/08/23 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - nn1773 [pii]
AID - 10.1038/nn1773 [doi]
PST - ppublish
SO  - Nat Neurosci. 2006 Oct;9(10):1265-73. doi: 10.1038/nn1773. Epub 2006 Sep 17.