PMID- 16981044
OWN - NLM
STAT- MEDLINE
DCOM- 20070503
LR  - 20190606
IS  - 0100-879X (Print)
IS  - 0100-879X (Linking)
VI  - 39
IP  - 9
DP  - 2006 Sep
TI  - Partial purification of tightly bound mitochondrial hexokinase from maize (Zea 
      mays L.) root membranes.
PG  - 1159-69
AB  - In mammals, hexokinase (HK) is strategically located at the outer membrane of 
      mitochondria bound to the porin protein. The mitochondrial HK is a crucial 
      modulator of apoptosis and reactive oxygen species generation. In plants, these 
      properties related to HK are unknown. In order to better understand the 
      physiological role of non-cytosolic hexokinase (NC-HK) in plants, we developed a 
      purification strategy here described. Crude extract of 400 g of maize roots (230 
      mg protein) contained a specific activity of 0.042 micromol G6P min(-1) mg 
      PTN(-1). After solubilization with detergent two fractions were obtained by DEAE 
      column chromatography, NC-HK 1 (specific activity = 3.6 micromol G6P min(-1) mg 
      PTN(-1) and protein recovered = 0.7 mg) and NC-HK 2. A major purification (yield 
      = 500-fold) was obtained after passage of NC-HK 1 through the hydrophobic 
      phenyl-Sepharose column. The total amount of protein and activity recovered were 
      0.04 and 18%, respectively. The NC-HK 1 binds to the hydrophobic phenyl-Sepharose 
      matrix, as observed for rat brain HK. Mild chymotrypsin digestion did not affect 
      adsorption of NC-HK 1 to the hydrophobic column as it does for rat HK I. In 
      contrast to mammal mitochondrial HK, glucose-6-phosphate, clotrimazole or 
      thiopental did not dissociate NC-HK from maize (Zea mays) or rice (Oryza sativa) 
      mitochondrial membranes. These data show that the interaction between maize or 
      rice NC-HK to mitochondria differs from that reported in mammals, where the 
      mitochondrial enzyme can be displaced by modulators or pharmacological agents 
      known to interfere with the enzyme binding properties with the mitochondrial 
      porin protein.
FAU - Rezende, G L
AU  - Rezende GL
AD  - Instituto de Bioquimica Medica, Centro de Ciencias da Saude, Universidade Federal 
      do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
FAU - Logullo, C
AU  - Logullo C
FAU - Meyer, L
AU  - Meyer L
FAU - Machado, L B
AU  - Machado LB
FAU - Oliveira-Carvalho, A L
AU  - Oliveira-Carvalho AL
FAU - Zingali, R B
AU  - Zingali RB
FAU - Cifuentes, D
AU  - Cifuentes D
FAU - Galina, A
AU  - Galina A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Braz J Med Biol Res
JT  - Brazilian journal of medical and biological research = Revista brasileira de 
      pesquisas medicas e biologicas
JID - 8112917
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Brain/enzymology
MH  - Chromatography, DEAE-Cellulose
MH  - Hexokinase/*isolation & purification/metabolism
MH  - Mitochondria/*enzymology
MH  - Oryza/enzymology
MH  - Plant Roots/*enzymology
MH  - Rats
MH  - Solubility
MH  - Zea mays/*enzymology
EDAT- 2006/09/19 09:00
MHDA- 2007/05/04 09:00
CRDT- 2006/09/19 09:00
PHST- 2005/12/14 00:00 [received]
PHST- 2006/05/18 00:00 [accepted]
PHST- 2006/09/19 09:00 [pubmed]
PHST- 2007/05/04 09:00 [medline]
PHST- 2006/09/19 09:00 [entrez]
AID - S0100-879X2006000900003 [pii]
AID - 10.1590/s0100-879x2006000900003 [doi]
PST - ppublish
SO  - Braz J Med Biol Res. 2006 Sep;39(9):1159-69. doi: 
      10.1590/s0100-879x2006000900003.