PMID- 16982432
OWN - NLM
STAT- MEDLINE
DCOM- 20080530
LR  - 20171213
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 26
IP  - 9
DP  - 2006 Sep
TI  - [Modulation of inflammatory cytokines by ATP-binding cassette A1 in THP-1 
      macrophages].
PG  - 1269-72
AB  - OBJECTIVE: To observe the effects of ATP-binding cassette A1 (ABCA1) on 
      intercellular cell adhesion molecule type 1 (ICAM-1), monocyte chemoattractant 
      protein-1 (MCP-1) and interleukin-1beta (IL-1beta) in THP-1 macrophages 
      stimulated with 8-Br-cAMP to identify a possible new mechanism that ABCA1 
      contributes to atherosclerogenesis (AS). METHODS: Monocytic THP-1 cells were 
      cultured in the presence of 100 nmol/L phorbol myristate acetate (PMA) for 72 h 
      to transform the cells into THP-1 macrophages. After the macrophages were 
      stimulated with 8-Br-cAMP (final concentration 0.5 mmol/L) for 3, 6, 12 and 24 h 
      respectively, the amounts of ABCA1, ICAM-1 and MCP-1 mRNA were examined by 
      real-time fluorescent quantitative RT-PCR, and the protein amounts of ABCA1, 
      ICAM-1, MCP-1 and IL-1beta were determined by Western blotting and enzyme-linked 
      immunosorbent assay (ELISA). Phosphorothioate antisense oligonucleotides of ABCA1 
      were add into the culture media at a final concentration of 100 nmol/L and the 
      experiments were repeated. RESULTS: ABCA1, ICAM-1 and MCP-1 mRNA and protein and 
      IL-1beta protein were increased in the macrophages after stimulation with 
      8-Br-cAMP for 6 and 12 h. The mRNA expressions of ABCA1, ICAM-1 and MCP-1 were 
      decreased significantly at 3 and 6 h (P<0.01), and the protein expressions of 
      ABCA1, ICAM-1, MCP-1 and IL-1beta declined significantly at 12 and 24 h (P<0.01) 
      after transfection of the macrophages with antisense oligonucleotides of ABCA1. 
      CONCLUSION: ABCA1 can increase the expressions of the inflammatory cytokines in 
      THP-1 macrophages stimulated by 8-Br-cAMP and plays a role in the pathogenesis of 
      AS.
FAU - Guo, Zhi-gang
AU  - Guo ZG
AD  - Department of Cardiology, Nanfang Hospital, Southern Medical University, 
      Guangzhou 510515, China. guodoctor@hotmail.com
FAU - Wu, Ping-sheng
AU  - Wu PS
FAU - Li, Jian-hua
AU  - Li JH
FAU - Lai, Wen-yan
AU  - Lai WY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - ATP Binding Cassette Transporter 1
MH  - ATP-Binding Cassette Transporters/biosynthesis/*genetics
MH  - Cell Line
MH  - Chemokine CCL2/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/*genetics/metabolism
MH  - Interleukin-1beta/genetics/metabolism
MH  - Macrophages/cytology/drug effects/*metabolism
MH  - Monocytes/cytology/drug effects/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2006/09/20 09:00
MHDA- 2008/05/31 09:00
CRDT- 2006/09/20 09:00
PHST- 2006/09/20 09:00 [pubmed]
PHST- 2008/05/31 09:00 [medline]
PHST- 2006/09/20 09:00 [entrez]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2006 Sep;26(9):1269-72.