PMID- 16982656
OWN - NLM
STAT- MEDLINE
DCOM- 20070129
LR  - 20220310
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 21
IP  - 11
DP  - 2006 Nov
TI  - Effects of ageing on spermatozoal chromatin and its sensitivity to in vivo and in 
      vitro oxidative challenge in the Brown Norway rat.
PG  - 2901-10
AB  - BACKGROUND: The goals of our study were to examine chromatin packaging and 
      integrity in spermatozoa taken from the caput and cauda epididymides of young 
      (4-month-old) and old (21-month-old) Brown Norway rats and to assess whether 
      spermatozoal sensitivity to oxidative treatments is altered with age. METHODS: 
      Oxidative treatments consisted of (i) in vivo oxidative challenge by systemic 
      administration of the glutathione-depleting drug l-buthionine-[S,R]-sulphoximine 
      (BSO) and (ii) in vitro oxidative challenge by incubating collected spermatozoa 
      with hydrogen peroxide (H(2)O(2)). Chromatin parameters assessed included 
      quantification of thiols, nuclear chromomycin A3 (CMA3) penetration, DNA breaks 
      by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling 
      (TUNEL) and ease of DNA dissociation by acridine orange (AO) staining. RESULTS: 
      In spermatozoa from older rats, we found decreases in thiols, CMA3 penetration 
      and the percentage of cells that undergo DNA dissociation. Administration of BSO 
      had oxidizing effects on the thiol groups. It also decreased CMA3 penetration and 
      DNA dissociation and increased TUNEL staining. Furthermore, BSO treatment 
      sensitized cauda epididymidis spermatozoa, from older animals, to H(2)O(2). 
      CONCLUSIONS: Overall, we show that spermatozoa from older rats have altered 
      chromatin packaging and integrity and that spermatozoa from the cauda 
      epididymidis are more responsive to combined in vivo and in vitro oxidative 
      challenge than spermatozoa from young rats.
FAU - Zubkova, Ekaterina V
AU  - Zubkova EV
AD  - Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, 
      Canada.
FAU - Robaire, Bernard
AU  - Robaire B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060918
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Chromatin)
RN  - 0 (Sulfhydryl Compounds)
RN  - 5072-26-4 (Buthionine Sulfoximine)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Buthionine Sulfoximine/*pharmacology
MH  - Chromatin/drug effects/*physiology
MH  - DNA Damage
MH  - Epididymis
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Inbred BN
MH  - Spermatozoa/drug effects/*physiology
MH  - Sulfhydryl Compounds/metabolism
EDAT- 2006/09/20 09:00
MHDA- 2007/01/30 09:00
CRDT- 2006/09/20 09:00
PHST- 2006/09/20 09:00 [pubmed]
PHST- 2007/01/30 09:00 [medline]
PHST- 2006/09/20 09:00 [entrez]
AID - del193 [pii]
AID - 10.1093/humrep/del193 [doi]
PST - ppublish
SO  - Hum Reprod. 2006 Nov;21(11):2901-10. doi: 10.1093/humrep/del193. Epub 2006 Sep 
      18.