PMID- 16984342
OWN - NLM
STAT- MEDLINE
DCOM- 20070305
LR  - 20071115
IS  - 0936-6768 (Print)
IS  - 0936-6768 (Linking)
VI  - 41
IP  - 5
DP  - 2006 Oct
TI  - Apoptosis-independent poor morphology of bovine embryos produced by multiple 
      ovulation.
PG  - 383-5
AB  - In multiple ovulation and embryo transfer (MOET) programmes in cattle, a 
      considerable number of morphologically poor-quality embryos continue to be 
      produced; this is one of the limiting factors of the technique. Apoptosis has 
      often been implicated in developmental arrest and fragmentation; these are 
      regarded as poor traits of embryonic quality in mammalian pre-implantation 
      embryos. In the present study, apoptosis was assessed in morphologically 
      poor-quality embryos in comparison with good-quality embryos that were recovered 
      from a MOET programme. Retarded embryos (two to 16 cell stage), morulae with 
      severe fragmentation and morphologically good-quality morulae recovered from 
      superstimulated cows at day 7 post-insemination were subjected to TdT-mediated 
      dUTP nick-end labelling (TUNEL) and Hoechst staining. Cell nuclei that showed 
      both TUNEL staining and apoptotic morphology were considered to be apoptotic. 
      Apoptotic index (AI) was calculated as the percentage of apoptotic cells per 
      embryo. Fifteen of 17 retarded embryos and 10 of 15 morphologically poor-quality 
      morulae did not show signs of apoptosis. The mean AIs in the morphologically 
      poor-quality embryos (two to 16 cell stage, 2.2%; poor morulae, 1.3%) were as low 
      as that in the good-quality embryos (2.9%). These results suggest that another 
      mode of developmental arrest and/or fragmentation that is independent of 
      apoptosis occurs in morphologically poor-quality embryos recovered from MOET 
      programmes.
FAU - Ikeda, S
AU  - Ikeda S
AD  - Livestock Farm of Graduate School of Agriculture, Kyoto University, Kyo-Tamba, 
      Kyoto Prefecture, Japan. shunta@kais.kyoto-u.ac.jp
FAU - Prendes, J M
AU  - Prendes JM
FAU - Alonso-Montes, C
AU  - Alonso-Montes C
FAU - Rodriguez, A
AU  - Rodriguez A
FAU - Diez, C
AU  - Diez C
FAU - Kitagawa, M
AU  - Kitagawa M
FAU - Imai, H
AU  - Imai H
FAU - Gomez, E
AU  - Gomez E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cattle/*embryology
MH  - Cell Nucleus/*ultrastructure
MH  - DNA Fragmentation
MH  - Embryo Transfer/*veterinary
MH  - Embryo, Mammalian/*cytology/pathology
MH  - Embryonic Development/physiology
MH  - In Situ Nick-End Labeling/veterinary
MH  - Morula/cytology/pathology
MH  - Superovulation/*physiology
EDAT- 2006/09/21 09:00
MHDA- 2007/03/06 09:00
CRDT- 2006/09/21 09:00
PHST- 2006/09/21 09:00 [pubmed]
PHST- 2007/03/06 09:00 [medline]
PHST- 2006/09/21 09:00 [entrez]
AID - RDA672 [pii]
AID - 10.1111/j.1439-0531.2006.00672.x [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2006 Oct;41(5):383-5. doi: 10.1111/j.1439-0531.2006.00672.x.