PMID- 16986209
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20191210
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 20
IP  - 20
DP  - 2006
TI  - Quantitative analyses of indoloquinazoline alkaloids in Fructus Evodiae by 
      high-performance liquid chromatography with atmospheric pressure chemical 
      ionization tandem mass spectrometry.
PG  - 3111-8
AB  - Fructus Evodiae (Wuzhuyu), the fruits of Evodia rutaecarpa and related varieties, 
      is widely used in traditional Chinese medicine. The bioactive constituents 
      include the indoloquinazoline alkaloids rutaecarpine, evodiamine and 
      dehydroevodiamine. A new assay based on high-performance liquid 
      chromatography/atmospheric pressure chemical ionization tandem mass spectrometry 
      (HPLC/UV/APCI-MS/MS) was developed for the measurement of the indoloquinazoline 
      alkaloids in commercial Fructus Evodiae products. Initially, the MS/MS 
      fragmentation pathways of indoloquinazoline alkaloids were investigated to 
      identify fragment ions that might be useful for the sensitive and selective 
      detection of trace indoloquinazoline alkaloids during LC/MS/MS. Then, 
      quantitative MS analysis of five indoloquinazoline alkaloids in 12 commercial 
      Fructus Evodiae products from different geographical sources was performed. 
      Analyte recovery was in the range of 97.5-105.3% for all with relative standard 
      deviations (RSDs) below 6%, the intra-assay and inter-assay RSDs were less than 
      7%, and good linear relationships were shown with correlation coefficients for 
      the analytes exceeding 0.999. Therefore, this LC/MS/MS assay facilitated the 
      rapid quantitative analysis of rutaecarpine, evodiamine, evodiamide, 
      14-formyldihydrorutaecarpine and dehydroevodiamine in 12 commercial Fructus 
      Evodiae products with excellent recovery, repeatability, accuracy and 
      sensitivity. This method is simple and specific and can be used for 
      identification and quality control of this traditional Chinese remedy.
CI  - Copyright 2006 John Wiley & Sons, Ltd.
FAU - Zhou, Yan
AU  - Zhou Y
AD  - Institute of Chinese Medicine and Department of Biology, The Chinese University 
      of Hong Kong, Shatin, Hong Kong, People's Republic of China.
FAU - Li, Sheng-Hong
AU  - Li SH
FAU - Jiang, Ren-Wang
AU  - Jiang RW
FAU - Cai, Min
AU  - Cai M
FAU - Liu, Xin
AU  - Liu X
FAU - Ding, Li-Sheng
AU  - Ding LS
FAU - Xu, Hong-Xi
AU  - Xu HX
FAU - But, Paul Pui-Hay
AU  - But PP
FAU - Shaw, Pang-Chui
AU  - Shaw PC
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Indoles)
RN  - 0 (Plant Extracts)
RN  - 0 (Quinazolines)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Evodia/*chemistry
MH  - Fruit/*chemistry
MH  - Indoles/*analysis
MH  - Plant Extracts/*chemistry
MH  - Quinazolines/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2006/09/21 09:00
MHDA- 2007/08/24 09:00
CRDT- 2006/09/21 09:00
PHST- 2006/09/21 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2006/09/21 09:00 [entrez]
AID - 10.1002/rcm.2705 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2006;20(20):3111-8. doi: 10.1002/rcm.2705.