PMID- 16987901
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20181113
IS  - 0007-1161 (Print)
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 91
IP  - 2
DP  - 2007 Feb
TI  - Intravitreal injection of Tacrolimus (FK506) suppresses ongoing experimental 
      autoimmune uveoretinitis in Rats.
PG  - 237-42
AB  - AIM: To determine whether intravitreal injection of tacrolimus suppresses ongoing 
      experimental autoimmune uveoretinitis (EAU) in rats. METHODS: Rats were immunised 
      with interphotoreceptor retinoid-binding protein peptide (R14) and given an 
      intravitreal injection of tacrolimus on day 12 after immunisation. Intraocular 
      inflammation was assessed by slit-lamp biomicroscopy and histopathological 
      examination. Interferon gamma and tumour necrosis factor alpha protein levels in 
      the ocular tissues were measured. Gene expression of chemokines was determined in 
      ocular tissues by reverse transcription-polymerase chain reaction. To evaluate 
      the systemic effect of intravitreal injection of tacrolimus, delayed-type 
      hypersensitivity was measured by ear swelling. RESULTS: Clinical and pathological 
      scores showed that ocular inflammation of tacrolimus-treated eyes was markedly 
      less than that of vehicle-treated eyes. The amount of interferon gamma and tumour 
      necrosis factor alpha was considerably inhibited in tacrolimus-treated eyes. The 
      gene expression of monocyte chemattractant protein-1 (MCP-1) and regulated upon 
      activation, normal T cell expressed and secreted (RANTES) was markedly reduced in 
      tacrolimus-treated eyes. Delayed-type hypersensitivity responses were not 
      impaired in tacrolimus-treated rats. CONCLUSIONS: Intravitreal injection of 
      tacrolimus was highly effective in suppressing the ongoing process of EAU without 
      any side effects on systemic cellular immunity. This treatment may be useful in 
      the management of patients with severe uveitis.
FAU - Oh-i, Keiko
AU  - Oh-i K
AD  - Department of Ophthalmology, Tokyo Medical University, 6-7-1, Nishi-shinjuku, 
      Shinjuku-ku, Tokyo 160-0023, Japan.
FAU - Keino, Hiroshi
AU  - Keino H
FAU - Goto, Hiroshi
AU  - Goto H
FAU - Yamakawa, Naoyuki
AU  - Yamakawa N
FAU - Murase, Kouhei
AU  - Murase K
FAU - Usui, Yoshihiko
AU  - Usui Y
FAU - Kezuka, Takeshi
AU  - Kezuka T
FAU - Sakai, Jun-Ichi
AU  - Sakai J
FAU - Takeuchi, Masaru
AU  - Takeuchi M
FAU - Usui, Masahiko
AU  - Usui M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060920
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
RN  - 0 (Chemokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
CIN - Br J Ophthalmol. 2007 Feb;91(2):135-6. PMID: 17244658
MH  - Animals
MH  - Autoimmune Diseases/*drug therapy/immunology/metabolism
MH  - Cells, Cultured
MH  - Chemokines/biosynthesis/genetics
MH  - Eye/metabolism
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Hypersensitivity, Delayed
MH  - Immunity, Cellular/drug effects
MH  - Immunosuppressive Agents/administration & dosage/adverse effects/*therapeutic use
MH  - Injections
MH  - Interferon-gamma/biosynthesis
MH  - Macrophages/drug effects/metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Retinitis/*drug therapy/immunology/metabolism
MH  - Tacrolimus/administration & dosage/*therapeutic use/toxicity
MH  - Treatment Outcome
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Uveitis/*drug therapy/immunology/metabolism
MH  - Vitreous Body
PMC - PMC1857637
COIS- Competing interests: None.
EDAT- 2006/09/22 09:00
MHDA- 2007/02/21 09:00
PMCR- 2010/02/01
CRDT- 2006/09/22 09:00
PHST- 2006/09/22 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/09/22 09:00 [entrez]
PHST- 2010/02/01 00:00 [pmc-release]
AID - bjo.2006.103168 [pii]
AID - bj103168 [pii]
AID - 10.1136/bjo.2006.103168 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2007 Feb;91(2):237-42. doi: 10.1136/bjo.2006.103168. Epub 2006 
      Sep 20.