PMID- 16987965
OWN - NLM
STAT- MEDLINE
DCOM- 20070212
LR  - 20240413
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 1
DP  - 2007 Jan
TI  - A protein encoded by the bovine herpesvirus 1 latency-related gene interacts with 
      specific cellular regulatory proteins, including CCAAT enhancer binding protein 
      alpha.
PG  - 59-67
AB  - Following acute infection, bovine herpesvirus 1 establishes latency in sensory 
      neurons of trigeminal ganglia (TG). Reactivation from latency occurs 
      periodically, resulting in the shedding of infectious virus. The latency-related 
      (LR) RNA is abundantly expressed in TG of latently infected calves, and the 
      expression of LR proteins is necessary for dexamethasone-induced reactivation 
      from latency. Previously published studies also identified an alternatively 
      spliced LR transcript which is abundantly expressed in TG at 7 days after 
      infection and has the potential to encode a novel LR fusion protein. Seven days 
      after infection is when extensive viral gene expression is extinguished in TG and 
      latency is established, suggesting that LR gene products influence the 
      establishment of latency. In this study, we used a bacterial two-hybrid assay to 
      identify cellular proteins that interact with the novel LR fusion protein. The LR 
      fusion protein interacts with two proteins that can induce apoptosis (Bid and 
      Cdc42) and with CCAAT enhancer binding protein alpha (C/EBP-alpha). Additional 
      studies confirmed that the LR fusion protein interacts with human or insect 
      C/EBP-alpha. C/EBP-alpha protein expression is induced in TG neurons of infected 
      calves and after dexamethasone-induced reactivation from latency. Wild-type 
      C/EBP-alpha, but not a DNA binding mutant of C/EBP-alpha, enhances plaque 
      formation in bovine cells. We hypothesize that interactions between the LR fusion 
      protein and C/EBP-alpha promote the establishment of latency.
FAU - Meyer, Florencia
AU  - Meyer F
AD  - Department of Veterinary and Biomedical Sciences, Nebraska Center for Virology, 
      University of Nebraska, Lincoln, NE 68503, USA.
FAU - Perez, Sandra
AU  - Perez S
FAU - Geiser, Vicki
AU  - Geiser V
FAU - Sintek, Mark
AU  - Sintek M
FAU - Inman, Melissa
AU  - Inman M
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
GR  - T32 AI060547/AI/NIAID NIH HHS/United States
GR  - 1P20RR15635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060920
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (latency-related protein, Bovine herpesvirus 1)
SB  - IM
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-alpha/*metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Drosophila Proteins/metabolism
MH  - Drosophila melanogaster
MH  - Gene Expression Regulation, Viral
MH  - Herpesvirus 1, Bovine/*genetics/metabolism/physiology
MH  - Humans
MH  - Mutation
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Trigeminal Ganglion/metabolism/virology
MH  - Two-Hybrid System Techniques
MH  - Viral Proteins/genetics/*metabolism
MH  - Virus Latency/genetics/*physiology
PMC - PMC1797275
EDAT- 2006/09/22 09:00
MHDA- 2007/02/13 09:00
PMCR- 2007/05/01
CRDT- 2006/09/22 09:00
PHST- 2006/09/22 09:00 [pubmed]
PHST- 2007/02/13 09:00 [medline]
PHST- 2006/09/22 09:00 [entrez]
PHST- 2007/05/01 00:00 [pmc-release]
AID - JVI.01171-06 [pii]
AID - 1171-06 [pii]
AID - 10.1128/JVI.01171-06 [doi]
PST - ppublish
SO  - J Virol. 2007 Jan;81(1):59-67. doi: 10.1128/JVI.01171-06. Epub 2006 Sep 20.