PMID- 16988077
OWN - NLM
STAT- MEDLINE
DCOM- 20061030
LR  - 20080121
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 14
IP  - 8
DP  - 2006 Aug
TI  - Mesenteric adipose tissue-derived monocyte chemoattractant protein-1 plays a 
      crucial role in adipose tissue macrophage migration and activation in obese mice.
PG  - 1353-62
AB  - OBJECTIVE: To determine whether chemokines, which play a pivotal role in 
      monocyte/macrophage trafficking, modulate macrophage infiltration into and 
      activation in the adipose tissues. RESEARCH METHODS AND PROCEDURES: Various types 
      of adipose tissue were isolated from different fat depots (e.g., mesenteric, 
      epididymal, renal, and subcutaneous adipose tissues) from obese mice fed a 
      high-fat diet and from non-obese controls fed a standard diet. The isolated 
      tissues were cultured for 24, 48, and 72 hours. The level of monocyte 
      chemoattractant protein-1 (MCP-1) expression and the amount of protein released 
      were measured by reverse transcriptase-polymerase chain reaction or enzyme-linked 
      immunosorbent assay, respectively. Chemotaxis assay was performed to measure the 
      degree of macrophage migration. Macrophage activation was estimated by measuring 
      the concentrations of nitric oxide and tumor necrosis factor alpha. RESULTS: The 
      level of MCP-1 mRNA expression, protein content, and the amount of protein 
      released significantly increased in the adipose tissues from the obese mice 
      compared with those from the non-obese mice. The mesenteric adipose tissue 
      produced the highest levels of MCP-1 protein among the four different fat depots. 
      Mesenteric adipose tissue-conditioned medium induced the highest degree of 
      macrophage migration and strongly induced macrophages to produce proinflammatory 
      mediators such as nitric oxide and tumor necrosis factor alpha. The 
      neutralization of MCP-1 in the adipose tissue-conditioned medium significantly 
      inhibited the migration and activation of macrophages. DISCUSSION: Our findings 
      suggest that MCP-1 plays a crucial role in adipose tissue inflammatory response 
      by activating and inducing the infiltration of macrophages into adipose tissues. 
      MCP-1 may be closely associated with visceral obesity-related complications and, 
      thus, may be a useful therapeutic target for modulating visceral obesity-related 
      diseases.
FAU - Yu, Rina
AU  - Yu R
AD  - Department of Food Science and Nutrition, University of Ulsan, Ulsan 680-749, 
      Korea. rinayu@ulsan.ac.kr
FAU - Kim, Chu-Sook
AU  - Kim CS
FAU - Kwon, Byung-Se
AU  - Kwon BS
FAU - Kawada, Teruo
AU  - Kawada T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (Antibodies)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Dietary Fats)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Cell Line
MH  - Chemokine CCL2/*genetics/immunology/metabolism
MH  - Chemotaxis/drug effects/physiology
MH  - Culture Media, Conditioned/pharmacology
MH  - Dietary Fats/administration & dosage
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Macrophage Activation/drug effects/physiology
MH  - Macrophages/cytology/drug effects/*physiology
MH  - Male
MH  - Mesentery/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Obesity/etiology/*physiopathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Culture Techniques
EDAT- 2006/09/22 09:00
MHDA- 2006/10/31 09:00
CRDT- 2006/09/22 09:00
PHST- 2006/09/22 09:00 [pubmed]
PHST- 2006/10/31 09:00 [medline]
PHST- 2006/09/22 09:00 [entrez]
AID - 14/8/1353 [pii]
AID - 10.1038/oby.2006.153 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2006 Aug;14(8):1353-62. doi: 10.1038/oby.2006.153.