PMID- 1698820
OWN - NLM
STAT- MEDLINE
DCOM- 19901121
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 86
IP  - 4
DP  - 1990 Oct
TI  - Modulation of interleukin-1 beta RNA in monocytic cells infected with human 
      immunodeficiency virus-1.
PG  - 1109-14
AB  - The effect of HIV-1 infection on cytokine levels was studied in monocytic cells 
      by using Northern blotting analysis. Monoblasts (THP-1, U937) did not express 
      IL-1 beta RNA even if the cells were infected with HIV-1. After exposure to LPS 
      (10 micrograms/ml) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 100 nM) for 12 
      h, these HIV-1-infected monoblasts accumulated 8-15-fold greater levels of IL-1 
      beta RNA as compared with their HIV-1-uninfected counterparts that were similarly 
      stimulated. In contrast, levels of RNAs coding for monocyte-colony-stimulating 
      factor (M-CSF) and tumor necrosis factor-alpha (TNF alpha) were elevated less 
      than twofold in the HIV-1-infected cells as compared with HIV-1-uninfected cells 
      after their stimulation with LPS and TPA. Inhibition of new protein synthesis did 
      not block the marked accumulation of IL-1 beta RNA produced by exposure to LPS 
      and TPA in the HIV-1-infected cells. Time-course experiments showed that the 
      maximal levels of IL-1 beta RNA occurred at 12 and 24 h after LPS and TPA 
      stimulation of the HIV-1-infected and uninfected U937 cells, respectively. 
      Studies of stability of RNA using actinomycin D showed that IL-1 beta RNA was 
      equally stable in infected and uninfected U937 cells after their stimulation with 
      TPA and LPS. Taken together, our data show that HIV-1 infection markedly augments 
      IL-1 beta RNA accumulation in stimulated monocytic cells, probably through 
      increasing rate of transcription of IL-1 beta.
FAU - Yamato, K
AU  - Yamato K
AD  - Department of Medicine, University of California School of Medicine, Los Angeles 
      90024-1678.
FAU - el-Hajjaoui, Z
AU  - el-Hajjaoui Z
FAU - Simon, K
AU  - Simon K
FAU - Koeffler, H P
AU  - Koeffler HP
LA  - eng
GR  - CA 26038/CA/NCI NIH HHS/United States
GR  - CA 33936/CA/NCI NIH HHS/United States
GR  - CA 43277/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 63231-63-0 (RNA)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cell Line
MH  - HIV Infections/*immunology
MH  - HIV-1/*physiology
MH  - Humans
MH  - Interleukin-1/biosynthesis/*genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/metabolism/*microbiology
MH  - RNA/*analysis
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC296839
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
PMCR- 1990/10/01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
PHST- 1990/10/01 00:00 [pmc-release]
AID - 10.1172/JCI114815 [doi]
PST - ppublish
SO  - J Clin Invest. 1990 Oct;86(4):1109-14. doi: 10.1172/JCI114815.