PMID- 16997466
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20061004
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 408
IP  - 1
DP  - 2006 Nov 6
TI  - Lin(-)CD34(-) bone marrow cells from adult mice can differentiate into 
      neural-like cells.
PG  - 51-6
AB  - Numerous studies have shown that some populations of bone marrow cells (BMCs) 
      have the capacity to differentiate into neural cells, which is useful for 
      repairing brain lesions. In this paper, we analyze neural differentiation 
      features of lineage-negative/CD34-negative (Lin(-)CD34(-)) cells in the bone 
      marrow of adult mice. The population of Lin(-)CD34(-) in BMCs was isolated by 
      magnetic bead sorting and fluorescence-activated cell sorter (FACS) using 
      specific lineage (CD4, CD8a, CD11b, CD45R, Gr-1 and TER-119) antibodies and CD34 
      antibody. First, we cultured Lin(-)CD34(-) BMCs in the presence of RNIF: vitamin 
      A derivative retinoic acid (RA) and neural-inducing factors (platelet-derived 
      growth factor BB (PDGF-BB), epidermal growth factor (EGF) and fibroblast growth 
      factor-basic (FGF-b)). Analyses of RT-PCR and immunocytochemistry indicated that 
      RNIF-treated Lin(-)CD34(-) BMCs expressed neural phenotypes as well as neurogenic 
      transcription factors. When we implanted the Lin(-)CD34(-) BMCs isolated from 
      enhanced green fluorescent protein (eGFP) transgenic mice into the subventricular 
      zone (SVZ) of postnatal mice, eGFP-positive cells survived 3 weeks after the 
      injection in the various brain regions, some of which expressed the neural 
      phenotypes. Our data suggest that certain subsets in the CD34(-) populations of 
      adult bone marrow could have the capacity to differentiate into neural cells in a 
      suitable environment.
FAU - Li, Qiang
AU  - Li Q
AD  - First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Osaka 570-8506, Japan.
FAU - Hosaka, Naoki
AU  - Hosaka N
FAU - Cui, Wenhao
AU  - Cui W
FAU - Wang, Xiaoli
AU  - Wang X
FAU - Cui, Yilong
AU  - Cui Y
FAU - Cui, Yunze
AU  - Cui Y
FAU - Song, Changye
AU  - Song C
FAU - Li, Qing
AU  - Li Q
FAU - Ryu, Takashi
AU  - Ryu T
FAU - Fan, Tianxue
AU  - Fan T
FAU - Kawamoto, Keiji
AU  - Kawamoto K
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
DEP - 20060925
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/genetics/*metabolism
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - *Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neurons/cytology/*physiology
EDAT- 2006/09/26 09:00
MHDA- 2007/04/04 09:00
CRDT- 2006/09/26 09:00
PHST- 2006/06/02 00:00 [received]
PHST- 2006/07/20 00:00 [revised]
PHST- 2006/08/11 00:00 [accepted]
PHST- 2006/09/26 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2006/09/26 09:00 [entrez]
AID - S0304-3940(06)00826-3 [pii]
AID - 10.1016/j.neulet.2006.08.063 [doi]
PST - ppublish
SO  - Neurosci Lett. 2006 Nov 6;408(1):51-6. doi: 10.1016/j.neulet.2006.08.063. Epub 
      2006 Sep 25.