PMID- 16998228
OWN - NLM
STAT- MEDLINE
DCOM- 20070104
LR  - 20061031
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 21
IP  - 6
DP  - 2006 Nov
TI  - Nitrocompound activation by cell-free extracts of nitroreductase-proficient 
      Salmonella typhimurium strains.
PG  - 369-74
AB  - A characterization of nitrocompounds activation by cell-free extracts (CFE) of 
      wild-type (AB(+)), SnrA deficient (B(+)), Cnr deficient (A(+)) and SnrA/Cnr 
      deficient (AB(-)) Salmonella typhimurium strains has been done. The Ames 
      mutagenicity test (S. typhimurium his(+) reversion assay) was used, as well as 
      nitroreductase (NR) activity determinations where the decrease in absorbance 
      generated by nitrofurantoin (NFN) reduction and NADP(H) oxidation in the presence 
      of NFN, nitrofurazone (NFZ), metronidazole (MTZ) and 4-nitroquinoline-1-oxide 
      (4NQO) were followed. Different aromatic and heterocyclic compounds were tested 
      for mutagenic activation: 2-nitrofluorene (2-NF); 2,7-dinitrofluorene (2,7-DNF); 
      1-nitropyrene (1-NP), 1,3-dinitropyrene (1,3-DNP); 1,6-dinitropyrene (1,6-DNP); 
      and 1,8-dinitropyrene (1,8-DNP). Differential mutagenicity was found with 
      individual cell free extracts, being higher when the wild type or Cnr containing 
      extract was used; nevertheless, depending on the nitrocompound, activation was 
      found when either NR, SnrA or Cnr, were present. In addition, all nitrocompounds 
      were more mutagenic after metabolic activation by CFE of NR proficient strains, 
      although AB(-) extract still showed activation capacity. On the other hand, NR 
      activity was predominantly catalyzed by wild type CFE followed by A(+), B(+) and 
      AB(-) extracts in that order. We can conclude that results from the Ames test 
      indicate that Cnr is the major NR, while NFN and NFZ reductions were 
      predominantly catalyzed by SnrA. The characterization of the residual NR activity 
      detected by the mutagenicity assay and the biochemical determinations in the 
      AB(-) CFE needs further investigation.
FAU - Salamanca-Pinzon, S G
AU  - Salamanca-Pinzon SG
AD  - Departamento de Medicina Genomica y Toxicologia Ambiental, Instituto de 
      Investigaciones Biomedicas Universidad Nacional Autonoma de Mexico, Mexico.
FAU - Camacho-Carranza, R
AU  - Camacho-Carranza R
FAU - Hernandez-Ojeda, S L
AU  - Hernandez-Ojeda SL
FAU - Espinosa-Aguirre, J J
AU  - Espinosa-Aguirre JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060923
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Mutagens)
RN  - 0 (Nitro Compounds)
RN  - EC 1.7.- (Nitroreductases)
SB  - IM
MH  - Biotransformation
MH  - Cell-Free System/metabolism
MH  - Mutagenicity Tests
MH  - Mutagens/toxicity
MH  - Nitro Compounds/*metabolism
MH  - Nitroreductases/*metabolism
MH  - Salmonella typhimurium/*enzymology/genetics
EDAT- 2006/09/26 09:00
MHDA- 2007/01/05 09:00
CRDT- 2006/09/26 09:00
PHST- 2006/09/26 09:00 [pubmed]
PHST- 2007/01/05 09:00 [medline]
PHST- 2006/09/26 09:00 [entrez]
AID - gel042 [pii]
AID - 10.1093/mutage/gel042 [doi]
PST - ppublish
SO  - Mutagenesis. 2006 Nov;21(6):369-74. doi: 10.1093/mutage/gel042. Epub 2006 Sep 23.