PMID- 16998648
OWN - NLM
STAT- MEDLINE
DCOM- 20070313
LR  - 20181113
IS  - 0340-5354 (Print)
IS  - 0340-5354 (Linking)
VI  - 253
IP  - 9
DP  - 2006 Sep
TI  - CSF protein profiling using Multiplex Immuno-assay: A potential new diagnostic 
      tool for leptomeningeal metastases.
PG  - 1177-84
AB  - OBJECTIVE: The diagnosis of leptomeningeal metastases (LM) is based on clinical 
      symptoms, magnetic resonance imaging (MRI) of brain and spine and cytological 
      analysis of cerebrospinal fluid (CSF). The clinical picture of LM is highly 
      variable and both cytological CSF analysis and contrast-enhanced MRI are limited 
      in sensitivity. More sensitive tools are needed to diagnose LM. We measured a 
      profile of proteins involved in adhesion and inflammation in the CSF of LM and 
      control patients and determined their potential diagnostic value for LM. PATIENTS 
      AND METHODS: Using Multiplex Immuno-Assay (MIA), the CSF concentrations of nine 
      soluble adhesion molecules, cyto- and chemokines were measured in patients with 
      cytologically proven LM (n = 57) and control patients with a systemic malignancy 
      (n = 20), aseptic/viral meningitis (n = 11) or other (non-)neurological diseases 
      (n = 19). RESULTS: We found high CSF levels of soluble Vascular Cell Adhesion 
      Molecule-1 (sVCAM-1), soluble Intercellular Adhesion Molecule-1 (sICAM-1), 
      Interleukin-8 (IL-8), Pulmonary and Activation Regulated Chemokine (PARC), 
      Interleukin-18 (IL-18) and Interferon-gamma inducible protein (IP-10) in patients 
      with LM. The CSF protein profile in LM patients differed significantly from the 
      profile found in control patients. Multivariate logistic regression and ROC 
      analysis showed that the MIA-measured CSF protein profile has an additive 
      discriminating value for LM above standard CSF parameters. A combination of total 
      protein, glucose, IL-8, PARC and IP-10 CSF levels proved to be most 
      discriminative between LM and non-LM patients. CONCLUSION: Our results warrant a 
      prospective study to determine whether a CSF protein profile, including IL-8, 
      PARC and IP-10 has diagnostic value compared with CSF cytology, the golden 
      standard for LM.
FAU - Brandsma, Dieta
AU  - Brandsma D
AD  - Department of Neurology, G03.228, University Medical Center Utrecht, PO Box 
      85500, 3584 Utrecht, CX, The Netherlands. d.brandsma@umcutrecht.nl
FAU - Voest, Emile E
AU  - Voest EE
FAU - de Jager, Wilco
AU  - de Jager W
FAU - Bonfrer, Hans
AU  - Bonfrer H
FAU - Algra, Ale
AU  - Algra A
FAU - Boogerd, Willem
AU  - Boogerd W
FAU - Korse, Tiny
AU  - Korse T
FAU - Reijneveld, Jaap C
AU  - Reijneveld JC
FAU - Verbeek, Marcel M
AU  - Verbeek MM
FAU - Rijkers, Ger
AU  - Rijkers G
FAU - Taphoorn, Martin J B
AU  - Taphoorn MJ
LA  - eng
PT  - Journal Article
DEP - 20060922
PL  - Germany
TA  - J Neurol
JT  - Journal of neurology
JID - 0423161
RN  - 0 (Cerebrospinal Fluid Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cerebrospinal Fluid Proteins/*metabolism
MH  - Female
MH  - Humans
MH  - Immunoassay/*methods
MH  - Logistic Models
MH  - Male
MH  - Meningeal Neoplasms/*cerebrospinal fluid/*secondary
MH  - Middle Aged
MH  - Neoplasm Metastasis/*diagnosis
MH  - ROC Curve
EDAT- 2006/09/26 09:00
MHDA- 2007/03/14 09:00
CRDT- 2006/09/26 09:00
PHST- 2005/07/19 00:00 [received]
PHST- 2005/12/13 00:00 [accepted]
PHST- 2006/09/26 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/09/26 09:00 [entrez]
AID - 10.1007/s00415-006-0187-y [doi]
PST - ppublish
SO  - J Neurol. 2006 Sep;253(9):1177-84. doi: 10.1007/s00415-006-0187-y. Epub 2006 Sep 
      22.