PMID- 16998790
OWN - NLM
STAT- MEDLINE
DCOM- 20061221
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 119
IP  - 12
DP  - 2006 Dec 15
TI  - Melanoma cells interfere with the interaction of dendritic cells with NK/LAK 
      cells.
PG  - 2861-9
AB  - Dendritic cells (DCs) and natural killer (NK) cells are key players at the 
      interface between innate resistance and acquired immunity. NK cells can induce DC 
      maturation, a differentiation process whereby DCs respond to a environmental 
      stimulus and acquire the ability of eliciting adaptive immunity. Conversely, 
      maturing DCs promote NK functions in vivo and in vitro. This interplay has 
      important consequences on the immune response to pathogens and possibly to 
      neoplastic cells. Here, we show that B16 melanoma cells actively modulate the 
      interaction between DCs derived from bone marrow precursors and NK/LAK cells 
      propagated from the spleen of C57BL/6 mice. DCs increased in a dose-dependent 
      manner the ability of NK/LAK cells to kill melanoma cells and to produce 
      cytokines. This activatory cross-talk entailed the production of IL-18 by DCs and 
      of IFN-gamma by NK/LAK cells. Melanoma cells were not a passive target of NK 
      activity; they regulated the outcome of the interaction between DCs and NK/LAK 
      cells, inhibiting the in vitro production of cytokines as effectively as the 
      genetic deletion of IL-18 or IFN-gamma. Interference with the NK/DC interaction 
      possibly represents a mechanism used by growing tumors to evade the immune 
      response.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Capobianco, Annalisa
AU  - Capobianco A
AD  - Department of Oncology, Cancer Immunotherapy and Gene Therapy Program, Clinical 
      Immunology Unit, H San Raffaele Scientific Institute & Vita-Salute San Raffaele 
      University, Milano, Italy.
FAU - Rovere-Querini, Patrizia
AU  - Rovere-Querini P
FAU - Rugarli, Claudio
AU  - Rugarli C
FAU - Manfredi, Angelo A
AU  - Manfredi AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Interleukin-18)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Cytotoxicity Tests, Immunologic
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Female
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-18/genetics/metabolism
MH  - Killer Cells, Lymphokine-Activated/cytology/immunology/*metabolism
MH  - Killer Cells, Natural/cytology/immunology/*metabolism
MH  - Male
MH  - Melanoma/immunology/*metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2006/09/26 09:00
MHDA- 2006/12/22 09:00
CRDT- 2006/09/26 09:00
PHST- 2006/09/26 09:00 [pubmed]
PHST- 2006/12/22 09:00 [medline]
PHST- 2006/09/26 09:00 [entrez]
AID - 10.1002/ijc.22279 [doi]
PST - ppublish
SO  - Int J Cancer. 2006 Dec 15;119(12):2861-9. doi: 10.1002/ijc.22279.