PMID- 16999846
OWN - NLM
STAT- MEDLINE
DCOM- 20070409
LR  - 20071115
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 135
IP  - 4
DP  - 2006 Nov
TI  - Characterisation of dic(9;20)(p11-13;q11) in childhood B-cell precursor acute 
      lymphoblastic leukaemia by tiling resolution array-based comparative genomic 
      hybridisation reveals clustered breakpoints at 9p13.2 and 20q11.2.
PG  - 492-9
AB  - Although the dic(9;20)(p11-13;q11) is a recurrent chromosomal abnormality in 
      paediatric B-cell precursor acute lymphoblastic leukaemia (BCP ALL), occurring in 
      approximately 2% of the cases, its molecular genetic consequences have not been 
      elucidated. In the present study, high-resolution genome-wide array-based 
      comparative genomic hybridisation (array-CGH) and fluorescence in situ 
      hybridisation (FISH) were used to characterise the 9p and 20q breakpoints (BPs) 
      in seven childhood BCP ALLs with dic(9;20), which was shown to be unbalanced in 
      all of them, resulting in loss of 9p13.2-pter. Five of the cases had loss of 
      20q11.2-qter, whereas two displayed gain of 20cen-pter. All BPs on 9p clustered 
      in a 1.5 Mb segment of the sub-band 9p13.2; in three of the cases, the 20q BPs 
      mapped to three adjacent clones covering a distance of 350 kb at 20q11.2. Thus, 
      the aberration should be designated dic(9;20)(p13.2;q11.2). One of the ALLs, 
      shown to have a complex dic(9;20), was further investigated by FISH, revealing a 
      rearrangement of the haemapoietic cell kinase isoform p61 (HCK) gene at 20q11. 
      The disruption of HCK may result in a fusion gene or in loss of function. 
      Unfortunately, lack of material precluded further analyses of HCK. Thus, it 
      remains to be elucidated whether dic(9;20)(p13.2;q11.2) leads to a chimaeric gene 
      or whether the functionally important outcome is loss of 9p and 20q material.
FAU - Schoumans, Jacqueline
AU  - Schoumans J
AD  - Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, 
      Sweden. jacqueline.schoumans@ki.se
FAU - Johansson, Bertil
AU  - Johansson B
FAU - Corcoran, Martin
AU  - Corcoran M
FAU - Kuchinskaya, Ekaterina
AU  - Kuchinskaya E
FAU - Golovleva, Irina
AU  - Golovleva I
FAU - Grander, Dan
AU  - Grander D
FAU - Forestier, Erik
AU  - Forestier E
FAU - Staaf, Johan
AU  - Staaf J
FAU - Borg, Ake
AU  - Borg A
FAU - Gustafsson, Britt
AU  - Gustafsson B
FAU - Blennow, Elisabeth
AU  - Blennow E
FAU - Nordgren, Ann
AU  - Nordgren A
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20060926
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Burkitt Lymphoma/*genetics
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Nucleic Acid Hybridization/methods
MH  - Oligonucleotide Array Sequence Analysis/methods
EDAT- 2006/09/27 09:00
MHDA- 2007/04/10 09:00
CRDT- 2006/09/27 09:00
PHST- 2006/09/27 09:00 [pubmed]
PHST- 2007/04/10 09:00 [medline]
PHST- 2006/09/27 09:00 [entrez]
AID - BJH6328 [pii]
AID - 10.1111/j.1365-2141.2006.06328.x [doi]
PST - ppublish
SO  - Br J Haematol. 2006 Nov;135(4):492-9. doi: 10.1111/j.1365-2141.2006.06328.x. Epub 
      2006 Sep 26.