PMID- 17002899
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20151119
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 67
IP  - 9
DP  - 2006 Sep
TI  - Human monoclonal antibodies as a tool for the detection of HLA class I 
      allele-specific expression loss in head-and-neck squamous cell carcinoma and 
      corresponding lymph node metastases.
PG  - 692-9
AB  - Human leukocyte antigen (HLA) expression is important for the elimination of 
      tumor cells by the immune system and immunotherapy. Activated T cells directed 
      against tumor-associated antigens are fully capable of recognizing and 
      eradicating neoplastic cells. Therefore, HLA expression loss is considered to be 
      a main factor in tumor development. We report for the first time HLA-A and HLA-B 
      allele-specific expression analysis by immunohistochemical staining of fresh 
      tumor tissue and 9 lymph node metastases of 15 patients with head-and-neck 
      squamous cell carcinoma. Heterogeneous HLA expression and HLA expression loss was 
      detected in 13 tumor patients. Approximately 50% of the tumors had 
      allele-specific expression loss, which would have remained undetected using HLA 
      monomorphic and locus-specific antibodies. In the majority of the patients with 
      head-and-neck squamous cell carcinoma, HLA allele-specific expression loss 
      differed between primary lesions and metastases. This is important for the 
      efficacy of immunotherapy in these patients. It can be concluded that it is 
      crucial to study HLA expression at the allele-specific level of primary lesions 
      and metastases. It increases and refines our knowledge of HLA expression loss in 
      tumorgenesis, which will improve the development of specific immunotherapy.
FAU - Koene, Geert
AU  - Koene G
AD  - Department of Pathology, University Medical Centre, Utrecht, the Netherlands.
FAU - Mulder, Arend
AU  - Mulder A
FAU - van der Ven, Kevin
AU  - van der Ven K
FAU - Eijsink, Chantal
AU  - Eijsink C
FAU - Franke, Marry
AU  - Franke M
FAU - Slootweg, Piet
AU  - Slootweg P
FAU - Claas, Frans
AU  - Claas F
FAU - Tilanus, Marcel
AU  - Tilanus M
LA  - eng
PT  - Journal Article
DEP - 20060714
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - *Antibodies, Monoclonal
MH  - Carcinoma, Squamous Cell/*metabolism/secondary
MH  - Female
MH  - Head and Neck Neoplasms/*metabolism/pathology
MH  - Histocompatibility Antigens Class I/*biosynthesis/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphatic Metastasis/*immunology
MH  - Male
MH  - Middle Aged
EDAT- 2006/09/28 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/09/28 09:00
PHST- 2006/03/06 00:00 [received]
PHST- 2006/06/06 00:00 [accepted]
PHST- 2006/09/28 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/09/28 09:00 [entrez]
AID - S0198-8859(06)00152-2 [pii]
AID - 10.1016/j.humimm.2006.06.001 [doi]
PST - ppublish
SO  - Hum Immunol. 2006 Sep;67(9):692-9. doi: 10.1016/j.humimm.2006.06.001. Epub 2006 
      Jul 14.