PMID- 17004918
OWN - NLM
STAT- MEDLINE
DCOM- 20070105
LR  - 20150513
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 24
IP  - 6
DP  - 2006 Sep
TI  - Brain-derived neurotrophic factor facilitates in vivo internalization of tetanus 
      neurotoxin C-terminal fragment fusion proteins in mature mouse motor nerve 
      terminals.
PG  - 1546-54
AB  - In a previous study it was reported that fusion proteins composed of the atoxic 
      C-terminal fragment of tetanus toxin (TTC) and green fluorescent protein or 
      beta-galactosidase (GFP-TTC and beta-gal-TTC, respectively) rapidly cluster at 
      motor nerve terminals of the mouse neuromuscular junction (NMJ). Because this 
      traffic involves presynaptic activity, probably via the secretion of active 
      molecules, we examined whether it is affected by brain-derived neurotrophic 
      factor (BDNF). Quantitative confocal microscopy and a fluorimetric assay for 
      beta-gal activity revealed that co-injecting BDNF and the fusion proteins 
      significantly increased the kinetics and amount of the proteins' localization at 
      the NMJ and their internalization by motor nerve terminals. The observed 
      increases were independent of synaptic vesicle recycling because BDNF did not 
      affect spontaneous quantal acetylcholine release. In addition, injecting 
      anti-BDNF antibody shortly before injecting GFP-TTC, and before co-injecting 
      GFP-TTC and BDNF, significantly reduced the fusion protein's localization at the 
      NMJ. Co-injecting GFP-TTC with neurotrophin-4 (NT-4) or glial-derived 
      neurotrophic factor (GDNF), but not with nerve growth factor, neurotrophin-3 or 
      ciliary neurotrophic factor, also significantly increased the fusion protein's 
      localization at the NMJ. Thus, TTC probes may use for their neuronal 
      internalization endocytic pathways normally stimulated by BDNF, NT-4 and GDNF 
      binding. Different tyrosine kinase receptors with similar signalling pathways are 
      activated by BDNF/NT-4 and GDNF binding. Thus, activated components of these 
      signalling pathways may be involved in the TTC probes' internalization, perhaps 
      by facilitating localization of receptors of TTC in specific membrane 
      microdomains or by recruiting various factors needed for internalization of TTC.
FAU - Roux, Sylvie
AU  - Roux S
AD  - CNRS, Institut de Neurobiologie Alfred Fessard, FRC2118, Laboratoire de 
      Neurobiologie Cellulaire et Moleculaire, UPR9040, 1 Avenue de la Terrasse, 91198 
      Gif sur Yvette, France.
FAU - Saint Cloment, Cecile
AU  - Saint Cloment C
FAU - Curie, Thomas
AU  - Curie T
FAU - Girard, Emmanuelle
AU  - Girard E
FAU - Miana Mena, Francisco-Javier
AU  - Miana Mena FJ
FAU - Barbier, Julien
AU  - Barbier J
FAU - Osta, Rosario
AU  - Osta R
FAU - Molgo, Jordi
AU  - Molgo J
FAU - Brulet, Philippe
AU  - Brulet P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Tetanus Toxin)
RN  - 0 (tetanus toxin fragment C)
RN  - 108688-71-7 (neurofilament protein H)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Axonal Transport/*drug effects/physiology
MH  - Brain-Derived Neurotrophic Factor/immunology/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fluorometry/methods
MH  - Green Fluorescent Proteins/metabolism
MH  - Mice
MH  - Microscopy, Confocal/methods
MH  - Motor Neurons/*drug effects
MH  - Neurofilament Proteins/metabolism
MH  - Neuromuscular Junction/*cytology
MH  - Peptide Fragments/*metabolism
MH  - Protein Transport/drug effects
MH  - Receptor, trkB/metabolism
MH  - Sciatic Nerve/drug effects/physiology
MH  - Tetanus Toxin/*metabolism
MH  - Time Factors
MH  - beta-Galactosidase/metabolism
EDAT- 2006/09/29 09:00
MHDA- 2007/01/06 09:00
CRDT- 2006/09/29 09:00
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/01/06 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
AID - EJN5030 [pii]
AID - 10.1111/j.1460-9568.2006.05030.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 Sep;24(6):1546-54. doi: 10.1111/j.1460-9568.2006.05030.x.