PMID- 17005175
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20190508
IS  - 0012-1606 (Print)
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 299
IP  - 2
DP  - 2006 Nov 15
TI  - TrkB/BDNF signaling regulates photoreceptor progenitor cell fate decisions.
PG  - 455-65
AB  - Neurotrophins, via activation of Trk receptor tyrosine kinases, serve as 
      mitogens, survival factors and regulators of arborization during retinal 
      development. Brain-derived neurotrophic factor (BDNF) and TrkB regulate neuronal 
      arborization and survival in late retinal development. However, TrkB is expressed 
      during early retinal development where its functions are unclear. To assess 
      TrkB/BDNF actions in the early chick retina, replication-incompetent retroviruses 
      were utilized to over-express a dominant negative truncated form of TrkB (trunc 
      TrkB), or BDNF and effects were assessed at E15. Clones expressing trunc TrkB 
      were smaller than controls, and proliferation and apoptosis assays suggest that 
      decreased clone size correlated with increased cell death when BDNF/TrkB 
      signaling was impaired. Analysis of clonal composition revealed that trunc TrkB 
      over-expression decreased photoreceptor numbers (41%) and increased cell numbers 
      in the middle third of the inner nuclear layer (INL) (23%). Conversely, BDNF 
      over-expression increased photoreceptor numbers (25%) and decreased INL numbers 
      (17%). Photoreceptors over-expressing trunc TrkB demonstrated no increase in 
      apoptosis nor abnormalities in lamination suggesting that TrkB activation is not 
      required for photoreceptor cell survival or migration. These studies suggest that 
      TrkB signaling regulates commitment to and/or differentiation of photoreceptor 
      cells from retinal progenitor cells, identifying a novel role for TrkB/BDNF in 
      regulating cell fate decisions.
FAU - Turner, Brian A
AU  - Turner BA
AD  - Department of Medicine, Weill Medical College of Cornell University, 1300 York 
      Avenue, Room C606, New York, NY 10021, USA.
FAU - Sparrow, Janet
AU  - Sparrow J
FAU - Cai, Bolin
AU  - Cai B
FAU - Monroe, Julie
AU  - Monroe J
FAU - Mikawa, Takashi
AU  - Mikawa T
FAU - Hempstead, Barbara L
AU  - Hempstead BL
LA  - eng
GR  - R01 NS030687-14/NS/NINDS NIH HHS/United States
GR  - R01 EY012951/EY/NEI NIH HHS/United States
GR  - NS30687/NS/NINDS NIH HHS/United States
GR  - EY12293/EY/NEI NIH HHS/United States
GR  - R01 EY012293-03/EY/NEI NIH HHS/United States
GR  - R01 EY012293-02/EY/NEI NIH HHS/United States
GR  - R01 NS030687/NS/NINDS NIH HHS/United States
GR  - R01 EY012293-01A1/EY/NEI NIH HHS/United States
GR  - R01 EY012951-11/EY/NEI NIH HHS/United States
GR  - R01 NS030687-13/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060814
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Protein Isoforms)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain-Derived Neurotrophic Factor/genetics/*physiology
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chick Embryo
MH  - Photoreceptor Cells, Vertebrate/*cytology/physiology
MH  - Protein Isoforms/physiology
MH  - Receptor, trkB/genetics/*physiology
MH  - Retina/*cytology/embryology/metabolism
MH  - Signal Transduction/*physiology
MH  - Stem Cells/*cytology/physiology
PMC - PMC2623246
MID - NIHMS13716
EDAT- 2006/09/29 09:00
MHDA- 2007/01/17 09:00
PMCR- 2009/01/13
CRDT- 2006/09/29 09:00
PHST- 2005/08/05 00:00 [received]
PHST- 2006/08/07 00:00 [revised]
PHST- 2006/08/09 00:00 [accepted]
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
PHST- 2009/01/13 00:00 [pmc-release]
AID - S0012-1606(06)01093-1 [pii]
AID - 10.1016/j.ydbio.2006.08.025 [doi]
PST - ppublish
SO  - Dev Biol. 2006 Nov 15;299(2):455-65. doi: 10.1016/j.ydbio.2006.08.025. Epub 2006 
      Aug 14.