PMID- 17005525
OWN - NLM
STAT- MEDLINE
DCOM- 20070703
LR  - 20061225
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 22
IP  - 1
DP  - 2007 Jan
TI  - Functional impairment of monocyte-derived dendritic cells in patients with severe 
      chronic kidney disease.
PG  - 128-38
AB  - BACKGROUND: Dendritic cells (DCs) are antigen-presenting cells that are pivotal 
      for the initiation of the primary immune response. Patients with chronic kidney 
      disease (CKD) with or without chronic intermittent haemodialysis (CIHD) show an 
      impaired immune response. Dysfunction of DCs may underlie this phenomenon. 
      METHODS: In this study, several different functions of monocyte-derived DCs 
      (moDC) of patients with CKD class IV-V (glomerular filtration rate <30 ml/min) 
      and patients on CIHD were studied in vitro and compared with age- and sex-matched 
      healthy volunteers. RESULTS: We demonstrate that, independent of the maturation 
      stimulus used, mature moDC from both groups of patients did not acquire the same 
      level of terminal differentiation as moDC from controls, as shown by analysis of 
      cell surface markers and the relative high macropinocytosis activity of moDC. The 
      stimulation of allogeneic T-cells by immature moDC and mature moDC did not differ 
      between patients and controls. However, in the presence of immature moDC or 
      antigen-loaded maturated moDC from patients, less proliferation of autologous 
      T-cells was observed in response to recall antigens. There was no difference 
      between moDC from controls and patients in their ability to activate naive 
      T-cells and to differentiate them into Th1 and Th2 cells. CONCLUSIONS: These 
      results show that the terminal differentiation of moDC in patients with severe 
      CKD is impaired. This impairment is not restricted to one maturation stimulus and 
      is independent of treatment with haemodialysis.
FAU - Verkade, Martijn Anton
AU  - Verkade MA
AD  - Erasmus Medical Center, Department of Internal Medicine, Division of Nephrology, 
      Bd336, Dr. Molewater plein 40, 3015 GD Rotterdam, The Netherlands.
FAU - van Druningen, Corne Johan
AU  - van Druningen CJ
FAU - Vaessen, Leonard Maria Bernardus
AU  - Vaessen LM
FAU - Hesselink, Dennis Adriaan
AU  - Hesselink DA
FAU - Weimar, Willem
AU  - Weimar W
FAU - Betjes, Michiel Gerardus Henricus
AU  - Betjes MG
LA  - eng
PT  - Journal Article
DEP - 20060927
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Differentiation
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immune System/metabolism
MH  - Kidney Diseases/blood/*pathology
MH  - Leukocytes/cytology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Monocytes/*cytology
MH  - T-Lymphocytes/metabolism
EDAT- 2006/09/29 09:00
MHDA- 2007/07/04 09:00
CRDT- 2006/09/29 09:00
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/07/04 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
AID - gfl519 [pii]
AID - 10.1093/ndt/gfl519 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2007 Jan;22(1):128-38. doi: 10.1093/ndt/gfl519. Epub 
      2006 Sep 27.