PMID- 17005665
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 23
DP  - 2006 Dec
TI  - Preferential translation of vesicular stomatitis virus mRNAs is conferred by 
      transcription from the viral genome.
PG  - 11733-42
AB  - Host protein synthesis is inhibited in cells infected with vesicular stomatitis 
      virus (VSV). It has been proposed that viral mRNAs are subjected to the same 
      inhibition but are predominantly translated because of their abundance. To 
      compare translation efficiencies of viral and host mRNAs during infection, we 
      used an enhanced green fluorescent protein (EGFP) reporter expressed from a 
      recombinant virus or from the host nucleus in stably transfected cells. 
      Translation efficiency of host-derived EGFP mRNA was reduced more than threefold 
      at eight hours postinfection, while viral-derived mRNA was translated around 
      sevenfold more efficiently than host-derived EGFP mRNA in VSV-infected cells. To 
      test whether mRNAs transcribed in the cytoplasm are resistant to shutoff of 
      translation during VSV infection, HeLa cells were infected with a recombinant 
      simian virus 5 (rSV5) that expressed GFP. Cells were then superinfected with VSV 
      or mock superinfected. GFP mRNA transcribed by rSV5 was not resistant to 
      translation inhibition during superinfection with VSV, indicating that 
      transcription in the cytoplasm is not sufficient for preventing translation 
      inhibition. To determine if cis-acting sequences in untranslated regions (UTRs) 
      were involved in preferential translation of VSV mRNAs, we constructed EGFP 
      reporters with VSV or control UTRs and measured the translation efficiency in 
      mock-infected and VSV-infected cells. The presence of VSV UTRs did not affect 
      mRNA translation efficiency in mock- or VSV-infected cells, indicating that VSV 
      mRNAs do not contain cis-acting sequences that influence translation. However, we 
      found that when EGFP mRNAs transcribed by VSV or by the host were translated in 
      vitro, VSV-derived EGFP mRNA was translated 22 times more efficiently than 
      host-derived EGFP mRNA. This indicated that VSV mRNAs do contain cis-acting 
      structural elements (that are not sequence based), which enhance translation 
      efficiency of viral mRNAs.
FAU - Whitlow, Zackary W
AU  - Whitlow ZW
AD  - Department of Biochemistry, Wake Forest University School of Medicine, 
      Winston-Salem, NC 27157, USA. zwhitlow@wfubmc.edu
FAU - Connor, John H
AU  - Connor JH
FAU - Lyles, Douglas S
AU  - Lyles DS
LA  - eng
GR  - R01 AI052304/AI/NIAID NIH HHS/United States
GR  - R01 AI 052304/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20060927
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - *Genome, Viral
MH  - HeLa Cells
MH  - Humans
MH  - Protein Biosynthesis/*physiology
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/metabolism
MH  - Transcription, Genetic/*physiology
MH  - Vesicular stomatitis Indiana virus/genetics/metabolism/*physiology
MH  - Viral Proteins/*biosynthesis
PMC - PMC1642595
EDAT- 2006/09/29 09:00
MHDA- 2007/01/17 09:00
PMCR- 2007/04/01
CRDT- 2006/09/29 09:00
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - JVI.00971-06 [pii]
AID - 0971-06 [pii]
AID - 10.1128/JVI.00971-06 [doi]
PST - ppublish
SO  - J Virol. 2006 Dec;80(23):11733-42. doi: 10.1128/JVI.00971-06. Epub 2006 Sep 27.