PMID- 17005928
OWN - NLM
STAT- MEDLINE
DCOM- 20070122
LR  - 20181113
IS  - 1556-6811 (Print)
IS  - 1556-679X (Electronic)
IS  - 1556-679X (Linking)
VI  - 13
IP  - 12
DP  - 2006 Dec
TI  - Quantitative and functional differences between peripheral blood myeloid 
      dendritic cells from patients with pleural and parenchymal lung tuberculosis.
PG  - 1299-306
AB  - Dendritic cells (DCs) play a pivotal role in generating protective host immunity 
      to Mycobacterium tuberculosis. Few studies have addressed DC function in the 
      context of active tuberculosis (TB), largely due to technical constraints in 
      obtaining adequate numbers of DC from sick patients. We quantitated peripheral 
      blood myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the whole blood of 
      patients with active TB and show that blood from patients with pleural TB was 
      characterized by high circulating levels of mDCs. We also developed and optimized 
      a novel whole-blood assay to study mDC production of the Th1-promoting cytokine 
      interleukin 12 (IL-12) and upregulation of the maturation marker CCR7 after 
      incubation with mycobacteria. We found that pleural TB was associated with 
      increased IL-12 production and CCR7 expression compared to lung parenchymal 
      disease. Our findings suggest important differences in innate immunity between 
      patients with different forms of active TB, and this may contribute to the 
      differences in natural history observed between the two groups.
FAU - Mendelson, Marc
AU  - Mendelson M
AD  - Laboratory of Mycobcterial Immunity and Pathogenesis, Public Health Research 
      Institute, International Center for Public Health, Newark, New Jersey 07103-3535, 
      USA. mmendels@curie.uct.ac.za
FAU - Hanekom, Willem A
AU  - Hanekom WA
FAU - Ntutela, Siyabulela
AU  - Ntutela S
FAU - Vogt, Monica
AU  - Vogt M
FAU - Steyn, Lafras
AU  - Steyn L
FAU - Maartens, Gary
AU  - Maartens G
FAU - Kaplan, Gilla
AU  - Kaplan G
LA  - eng
GR  - R01 AI054361/AI/NIAID NIH HHS/United States
GR  - AI 65653/AI/NIAID NIH HHS/United States
GR  - R01 AI065653/AI/NIAID NIH HHS/United States
GR  - AI 22616/AI/NIAID NIH HHS/United States
GR  - AI 54361/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20060927
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (CCR7 protein, human)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, Chemokine)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adult
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Immunity, Innate
MH  - Interleukin-12/metabolism
MH  - Male
MH  - Middle Aged
MH  - Myeloid Cells/cytology/*immunology
MH  - Receptors, CCR7
MH  - Receptors, Chemokine/metabolism
MH  - Tuberculosis, Pleural/*immunology
MH  - Tuberculosis, Pulmonary/*immunology
PMC - PMC1694453
EDAT- 2006/09/29 09:00
MHDA- 2007/01/24 09:00
PMCR- 2007/04/01
CRDT- 2006/09/29 09:00
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - CVI.00132-06 [pii]
AID - 0132-06 [pii]
AID - 10.1128/CVI.00132-06 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2006 Dec;13(12):1299-306. doi: 10.1128/CVI.00132-06. Epub 
      2006 Sep 27.