PMID- 17005981
OWN - NLM
STAT- MEDLINE
DCOM- 20070124
LR  - 20201109
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 152
IP  - Pt 10
DP  - 2006 Oct
TI  - Ecophysiology of different filamentous Alphaproteobacteria in industrial 
      wastewater treatment plants.
PG  - 3003-3012
LID - 10.1099/mic.0.29249-0 [doi]
AB  - The ecophysiology of five filamentous species affiliated to the 
      Alphaproteobacteria was investigated in industrial activated sludge systems. The 
      five species, 'Candidatus Alysiosphaera europaea', 'Candidatus Monilibacter 
      batavus', 'Candidatus Alysiomicrobium bavaricum', 'Candidatus Sphaeronema 
      italicum' and Meganema perideroedes, are very abundant in industrial wastewater 
      treatment plants and are often involved in bulking incidents. The morphology of 
      these filamentous bacterial species resembled Eikelboom's Nostocoida limicola, or 
      Type 021N, and could only be correctly identified by using fluorescence in situ 
      hybridization (FISH), applying species-specific gene probes. Two physiological 
      groupings of the five species were found using microautoradiography combined with 
      FISH. Group 1 ('Ca. Monilibacter batavus' and 'Ca. Sphaeronema italicum') 
      utilized many short-chained fatty acids (acetate, pyruvate and propionate), 
      whereas Group 2 ('Ca. Alysiosphaera europaea', 'Ca. Alysiomicrobium bavaricum' 
      and Meganema perideroedes) could also exploit several sugars, amino acids and 
      ethanol. All species had polyhydroxyalkanoate granules present and several of the 
      species had a very large storage capacity. No activity was found under strict 
      anaerobic conditions, while uptake of substrate was observed in the presence of 
      nitrate or nitrite as potential electron acceptor. However, for all species a 
      reduced number of substrates could be consumed under these conditions compared to 
      aerobic conditions. Only a little exo-enzymic activity was found and nearly all 
      species had a hydrophobic cell surface. Based on knowledge of the 
      ecophysiological potential, control strategies are suggested.
FAU - Kragelund, Caroline
AU  - Kragelund C
AD  - Section of Environmental Engineering, Department of Biotechnology, Chemistry and 
      Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 
      Aalborg, Denmark.
FAU - Kong, Yunhong
AU  - Kong Y
AD  - Section of Environmental Engineering, Department of Biotechnology, Chemistry and 
      Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 
      Aalborg, Denmark.
FAU - van der Waarde, Jaap
AU  - van der Waarde J
AD  - BIOCLEAR BV, Rozenburglaan 13, 9727 DL Groningen, The Netherlands.
FAU - Thelen, Karin
AU  - Thelen K
AD  - VERMICON AG, Emmy-Noether-Str. 2, 80992 Munchen, Germany.
FAU - Eikelboom, Dick
AU  - Eikelboom D
AD  - ASIS vof, Deventerweg 38, 7203 AK Zutphen, The Netherlands.
FAU - Tandoi, Valter
AU  - Tandoi V
AD  - CNR, Water Research Institute, Via Reno 1, 00198 Rome, Italy.
FAU - Thomsen, Trine Rolighed
AU  - Thomsen TR
AD  - Section of Environmental Engineering, Department of Biotechnology, Chemistry and 
      Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 
      Aalborg, Denmark.
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
AD  - Section of Environmental Engineering, Department of Biotechnology, Chemistry and 
      Environmental Engineering, Aalborg University, Sohngaardsholmsvej 57, DK-9000 
      Aalborg, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Amino Acids)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Enzymes)
RN  - 0 (Fatty Acids)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Polymers)
RN  - 0 (Sewage)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Aerobiosis
MH  - Alphaproteobacteria/classification/cytology/genetics/*physiology
MH  - Amino Acids/metabolism
MH  - Anaerobiosis
MH  - Bacterial Proteins/analysis
MH  - Bacterial Typing Techniques
MH  - Carbohydrate Metabolism
MH  - Cytoplasmic Granules/chemistry
MH  - Ecosystem
MH  - Enzymes/analysis
MH  - Ethanol/metabolism
MH  - Fatty Acids/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrates/metabolism
MH  - Nitrites/metabolism
MH  - Polymers/analysis
MH  - Sewage/*microbiology
MH  - *Water Microbiology
MH  - *Water Purification
EDAT- 2006/09/29 09:00
MHDA- 2007/01/25 09:00
CRDT- 2006/09/29 09:00
PHST- 2006/09/29 09:00 [pubmed]
PHST- 2007/01/25 09:00 [medline]
PHST- 2006/09/29 09:00 [entrez]
AID - 10.1099/mic.0.29249-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2006 Oct;152(Pt 10):3003-3012. doi: 
      10.1099/mic.0.29249-0.