PMID- 17007437
OWN - NLM
STAT- MEDLINE
DCOM- 20061026
LR  - 20190902
IS  - 0015-5632 (Print)
IS  - 0015-5632 (Linking)
VI  - 51
IP  - 4
DP  - 2006
TI  - Distribution of bifidobacteria in the gastrointestinal tract of calves.
PG  - 325-8
AB  - Development of gastrointestinal microflora of calves with special reference to 
      bifidobacteria was investigated; fecal bacteria were enumerated in calves aged 3 
      days to 7 weeks. Bacteria were detected by using selective media, bifidobacteria 
      using modified TPY agar with an addition of mupirocin and acetic acid and by 
      fluorescence in situ hybridization (FISH). Bifidobacteria were dominant group of 
      fecal flora of calves after 7 d of life, constituting 10 % of total bacterial 
      counts. The highest bacterial concentrations were observed in rumen, cecum, and 
      colon, the lowest in abomasum and duodenum. Bifidobacteria and lactobacilli 
      exhibited the highest survival ability during stomach passage and dominated in 
      all parts of the digestive tract. Bifidobacteria counts determined by FISH were 
      significantly higher than those provided by cultivation. Modified TPY agar was 
      highly selective and suitable for bifidobacteria isolation but FISH was shown to 
      be a more precise method for their enumeration. Our results show that 
      gastrointestinal microflora of calves in the milk-feeding period is similar to 
      breast-fed infants with respect to the occurrence of bifidobacteria as a dominant 
      bacterial group. The use of Bifidobacterium strains offers a promising way for 
      providing beneficial effectors for calves in the milk-feeding period.
FAU - Vlkova, E
AU  - Vlkova E
AD  - Department of Microbiology, Nutrition and Dietetics, Czech University of 
      Agriculture Prague, Czechia. vlkova@af.czu.cz
FAU - Trojanova, I
AU  - Trojanova I
FAU - Rada, V
AU  - Rada V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
SB  - IM
MH  - Animals
MH  - Animals, Suckling/*microbiology
MH  - Bifidobacterium/genetics/*growth & development/*isolation & purification
MH  - Cattle
MH  - Colony Count, Microbial
MH  - Ecosystem
MH  - Feces/microbiology
MH  - Gastrointestinal Tract/*microbiology
MH  - In Situ Hybridization, Fluorescence
EDAT- 2006/09/30 09:00
MHDA- 2006/10/27 09:00
CRDT- 2006/09/30 09:00
PHST- 2006/09/30 09:00 [pubmed]
PHST- 2006/10/27 09:00 [medline]
PHST- 2006/09/30 09:00 [entrez]
AID - 10.1007/BF02931825 [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 2006;51(4):325-8. doi: 10.1007/BF02931825.