PMID- 1700904
OWN - NLM
STAT- MEDLINE
DCOM- 19901228
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 172
IP  - 3
DP  - 1990 Nov 15
TI  - Isolation and characterization of a novel peptide amide from porcine brain.
PG  - 1167-74
AB  - Peptide with C-terminal tyrosine amide was isolated from porcine brain by acid 
      extraction and sequential steps of reverse phase HPLC. Microsequence, amino acid 
      and mass spectral analyses revealed the structure: 
      Ac-Ala-Ser-Glu-Lys-Arg-Pro-Ser-Glu-Arg-His-Gly-Ser-Lys- Tyr-amide. Since this 
      peptide had the identical sequence to N-terminus of porcine myelin basic protein 
      (pMBP) 1-14, we have designated porcine myelin peptide amide 14 (pMPA14). The 
      final HPLC step yielded 20 micrograms of homogeneous peptide preparation from 20 
      kg brain tissue. Unlike other amidated peptides, pMPA14 may be produced by non 
      enzymatic mechanism or unknown amidating enzyme. This unique amidation seems to 
      occur exclusively to MBP in the brain.
FAU - Takamatsu, K
AU  - Takamatsu K
AD  - Department of Psychiatry and Behavioral Sciences Stanford University School of 
      Medicine CA 94305.
FAU - Tatemoto, K
AU  - Tatemoto K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Peptide Fragments)
RN  - 0 (myelin basic protein 1-14)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Brain Chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Mass Spectrometry
MH  - Molecular Sequence Data
MH  - Myelin Basic Protein/chemistry/*isolation & purification
MH  - Peptide Fragments/*isolation & purification
MH  - Swine
EDAT- 1990/11/15 00:00
MHDA- 1990/11/15 00:01
CRDT- 1990/11/15 00:00
PHST- 1990/11/15 00:00 [pubmed]
PHST- 1990/11/15 00:01 [medline]
PHST- 1990/11/15 00:00 [entrez]
AID - 0006-291X(90)91571-9 [pii]
AID - 10.1016/0006-291x(90)91571-9 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1990 Nov 15;172(3):1167-74. doi: 
      10.1016/0006-291x(90)91571-9.