PMID- 17009257
OWN - NLM
STAT- MEDLINE
DCOM- 20061124
LR  - 20131121
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 54
IP  - 10
DP  - 2006 Oct
TI  - The role of fibroblasts and fibroblast-derived factors in periprosthetic 
      osteolysis.
PG  - 3221-32
AB  - OBJECTIVE: This study was undertaken to investigate how fibroblasts respond to 
      stimulation with particulate wear debris and/or conditioned media obtained from 
      pathologic tissue, and whether these activated fibroblasts express compounds that 
      are involved in bone resorption. METHODS: Conditioned media from explant cultures 
      of synovial tissue, periprosthetic soft tissue (interface membranes), titanium 
      particles, and proinflammatory cytokines were used to stimulate fibroblasts. 
      RNase protection assay was used to measure altered gene expression, and 
      enzyme-linked immunosorbent assay, Western blot hybridization, and flow cytometry 
      were used to determine fibroblast protein expression. Tartrate-resistant acid 
      phosphatase staining was used to identify multinucleated osteoclast-like cells. 
      RESULTS: The most dominant compounds measured in the conditioned media from 
      interface membranes were tumor necrosis factor alpha (TNFalpha), monocyte 
      chemoattractant protein 1 (MCP-1), interleukin-1beta (IL-1beta), IL-6, IL-8, and 
      vascular endothelial growth factor. Fibroblasts phagocytosed particulate wear 
      debris and responded to cytokine/chemokine stimulation. The most prominent 
      up-regulated genes and proteins secreted by fibroblasts in response to 
      stimulation were matrix metalloproteinase 1, MCP-1, IL-1beta, IL-6, IL-8, 
      cyclooxygenase 1 (COX-1), COX-2, leukemia inhibitory factor 1, transforming 
      growth factor beta1 (TGFbeta1), and TGFbeta receptor type I. In addition, 
      interface membrane fibroblasts expressed RANKL and osteoprotegerin in response to 
      stimulation with conditioned media, TNFalpha, or IL-1beta. Stimulated fibroblasts 
      cocultured with bone marrow cells in the presence of macrophage 
      colony-stimulating factor induced osteoclastogenesis. CONCLUSION: Interface 
      membrane fibroblasts respond directly to particulate wear debris, possibly via 
      phagocytosis, expressing proinflammatory cytokines and RANKL. Thus, these cells 
      may be actively involved in osteoclastogenesis and pathologic (periprosthetic) 
      bone resorption.
FAU - Koreny, Tamas
AU  - Koreny T
AD  - Rush University Medical Center, Chicago, Illinois 60612, USA.
FAU - Tunyogi-Csapo, Miklos
AU  - Tunyogi-Csapo M
FAU - Gal, Istvan
AU  - Gal I
FAU - Vermes, Csaba
AU  - Vermes C
FAU - Jacobs, Joshua J
AU  - Jacobs JJ
FAU - Glant, Tibor T
AU  - Glant TT
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (TNFSF11 protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Arthroplasty, Replacement, Knee/*adverse effects
MH  - Bone Resorption/genetics/metabolism/pathology/physiopathology
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytokines/genetics/*metabolism
MH  - Female
MH  - Fibroblasts/drug effects/*metabolism/*pathology
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Humans
MH  - Knee Prosthesis/*adverse effects
MH  - Macrophage Colony-Stimulating Factor/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Osteoclasts/drug effects/pathology
MH  - Osteolysis/*etiology/*metabolism/pathology
MH  - Osteoprotegerin/genetics/metabolism
MH  - Phagocytosis
MH  - RANK Ligand/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Titanium/pharmacology
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2006/09/30 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/09/30 09:00
PHST- 2006/09/30 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/09/30 09:00 [entrez]
AID - 10.1002/art.22134 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2006 Oct;54(10):3221-32. doi: 10.1002/art.22134.