PMID- 17010313
OWN - NLM
STAT- MEDLINE
DCOM- 20061204
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 350
IP  - 2
DP  - 2006 Nov 17
TI  - Increased matrix metalloproteinase-3 mRNA expression in visceral fat in mice 
      implanted with cultured preadipocytes.
PG  - 392-8
AB  - Using preadipocyte implantation methods, we recently demonstrated that adipocytes 
      in the visceral area change their function, as the expression of tumor necrosis 
      factor-alpha (TNF-alpha) increases, thereby causing insulin resistance. In order 
      to clarify the mechanism for changes in the function of adipocytes in visceral 
      area, we examined the mRNA expression profiles in visceral fat tissue specimens. 
      Four weeks after cell implantation, we performed a microarray analysis using the 
      RNA of fat tissue specimens implanted either with 3T3-L1 cells or PBS alone. 
      Sixty-three genes were thus isolated and the expression of matrix 
      metalloproteinase-3 (MMP-3) mRNA was found to dramatically increase in the fat 
      tissue specimens. The neutralization of MMP-3 protein inhibited adipogenesis and 
      the free fatty acid-induced TNF-alpha secretion in 3T3-L1 adipocytes. These 
      results suggest a potential role of MMP-3, which promotes the TNF-alpha 
      secretion, thus contributing to the disturbance of the functions in the 
      adipocytes which accumulate in the visceral area.
FAU - Unoki, Hiroyuki
AU  - Unoki H
AD  - Division of Applied Translational Research, Graduate School of Medicine, Chiba 
      University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
FAU - Bujo, Hideaki
AU  - Bujo H
FAU - Shibasaki, Manabu
AU  - Shibasaki M
FAU - Saito, Yasushi
AU  - Saito Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060920
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*enzymology/metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - Disease Models, Animal
MH  - Fatty Acids, Nonesterified/pharmacology
MH  - Gene Expression Regulation
MH  - Glucose/pharmacology
MH  - Insulin Resistance
MH  - Intra-Abdominal Fat/*enzymology/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/*biosynthesis/genetics
MH  - Matrix Metalloproteinase Inhibitors
MH  - Mice
MH  - Mice, Nude
MH  - Obesity/enzymology
MH  - RNA, Messenger/biosynthesis
MH  - Stem Cell Transplantation
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2006/10/03 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/10/03 09:00
PHST- 2006/09/11 00:00 [received]
PHST- 2006/09/12 00:00 [accepted]
PHST- 2006/10/03 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/03 09:00 [entrez]
AID - S0006-291X(06)02079-1 [pii]
AID - 10.1016/j.bbrc.2006.09.054 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2006 Nov 17;350(2):392-8. doi: 
      10.1016/j.bbrc.2006.09.054. Epub 2006 Sep 20.