PMID- 17010508
OWN - NLM
STAT- MEDLINE
DCOM- 20070504
LR  - 20220317
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 51
IP  - 2
DP  - 2007 Feb
TI  - Utility of fluorescence in situ hybridization as a non-invasive technique in the 
      diagnosis of upper urinary tract urothelial carcinoma.
PG  - 409-15; discussion 415
AB  - OBJECTIVES: To assess the clinical utility of a fluorescence in situ 
      hybridization (FISH) assay as a non-invasive method for diagnosing and monitoring 
      urothelial carcinoma (UC) in the upper urinary tract (UUT). METHODS: Urine 
      specimens from 30 consecutive patients with UUT UC and 19 healthy controls were 
      analyzed by means of cytology and FISH. For FISH analysis, labelled probes to 
      chromosomes 3, 7, 9, and 17 were used to assess chromosomal abnormalities 
      indicative of malignancy. Sensitivity and specificity of both techniques were 
      determined and compared. The frequency of chromosomal aberrations of malignant 
      cells from UUT was also determined. RESULTS: Overall sensitivity for FISH was 
      significantly higher than the corresponding value for urine cytology (76.7% vs. 
      36%, respectively, p=0.0056). Specificities for FISH and cytology were 94.7% and 
      100%, respectively (p=ns). The positive and negative predictive values for FISH 
      were 95.8% and 72%, whereas for cytology they were 100% and 54%, respectively. Of 
      the genetically altered nuclei counted, 67%, 54%, and 43% presented polysomy in 
      chromosomes 3, 7, and 17, respectively, and 21% presented a homozygous deletion 
      of chromosome 9. CONCLUSIONS: FISH assay of chromosomes 3, 7, 9, and 17 performed 
      on exfoliated cells from voided urine specimens has greater sensitivity than 
      cytology for detecting UUT UC whilst maintaining a similar specificity. The 
      non-invasive nature of this method and its higher sensitivity could contribute to 
      improving the current diagnosis of UUT UC.
FAU - Marin-Aguilera, Mercedes
AU  - Marin-Aguilera M
AD  - Department of Urology, Hospital Clinic, Institut d'Investigacions Biomediques 
      August Pi i Sunyer, Universitat de Barcelona, Barcelona, Spain.
FAU - Mengual, Lourdes
AU  - Mengual L
FAU - Ribal, Maria Jose
AU  - Ribal MJ
FAU - Musquera, Mireia
AU  - Musquera M
FAU - Ars, Elisabet
AU  - Ars E
FAU - Villavicencio, Humberto
AU  - Villavicencio H
FAU - Algaba, Ferran
AU  - Algaba F
FAU - Alcaraz, Antonio
AU  - Alcaraz A
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060908
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
SB  - IM
CIN - Eur Urol. 2007 Jul;52(1):288-9; author reply 289-91. PMID: 17383079
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*diagnosis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*diagnosis
MH  - *Kidney Pelvis
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Ureteral Neoplasms/*diagnosis
EDAT- 2006/10/03 09:00
MHDA- 2007/05/05 09:00
CRDT- 2006/10/03 09:00
PHST- 2006/05/15 00:00 [received]
PHST- 2006/08/22 00:00 [accepted]
PHST- 2006/10/03 09:00 [pubmed]
PHST- 2007/05/05 09:00 [medline]
PHST- 2006/10/03 09:00 [entrez]
AID - S0302-2838(06)01008-6 [pii]
AID - 10.1016/j.eururo.2006.08.045 [doi]
PST - ppublish
SO  - Eur Urol. 2007 Feb;51(2):409-15; discussion 415. doi: 
      10.1016/j.eururo.2006.08.045. Epub 2006 Sep 8.