PMID- 1701186
OWN - NLM
STAT- MEDLINE
DCOM- 19910117
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 39
IP  - 1
DP  - 1991 Jan
TI  - Flow cytometric detection of erythropoietic cytotoxicity in mouse bone marrow.
PG  - 15-21
AB  - Erythroblast proliferation and maturation in bone marrow are the processes 
      leading to the formation of polychromatic erythrocytes (PE) and normochromatic 
      erythrocytes (NE), respectively. PE contain RNA but no DNA, and can therefore be 
      distinguished both from NE (which lack both RNA and DNA) and from nucleated cells 
      (which contain both DNA and RNA). Cytotoxic agents that induce impairment of the 
      maturation process change the PE:NE ratio. We have developed a simple and rapid 
      method of determining the PE:NE ratio, based on flow cytometric analysis of 
      formaldehyde-fixed, acridine orange (AO)-stained cells. The effects of 
      cyclophosphamide (CP), mitomycin C (MMC), and vincristine (VC) were tested and 
      the PE:NE ratio was evaluated over 7 days of treatment. In this study we 
      monitored the kinetics of these compounds and were able to demonstrate both a 
      time- and a dose-dependent effect. We detected a difference between the effects 
      of the alkylating agents tested and those induced by the spindle inhibitor 
      tested. Flow cytometry of fixed bone marrow samples stained with AO provides more 
      information, better and more rapid statistical analysis, than conventional 
      microscopic methods for counting the PE:NE ratio.
FAU - Benning, V M
AU  - Benning VM
AD  - Rhone-Poulenc Sante, Centre de Recherche de Vitry-Alfortville, Institut de 
      Recherche sur la Securite du Medicament, Vitry sur Seine, France.
FAU - Maratrat, M B
AU  - Maratrat MB
FAU - Fournier, E C
AU  - Fournier EC
FAU - Melcion, C P
AU  - Melcion CP
FAU - Cordier, A C
AU  - Cordier AC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Mitomycins)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 50SG953SK6 (Mitomycin)
RN  - 5J49Q6B70F (Vincristine)
RN  - 63231-63-0 (RNA)
RN  - 8N3DW7272P (Cyclophosphamide)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - *Bone Marrow Cells
MH  - Cell Separation
MH  - Cell Survival/drug effects
MH  - Cyclophosphamide/*pharmacology
MH  - DNA/metabolism
MH  - Erythropoiesis/*drug effects
MH  - *Flow Cytometry
MH  - Formaldehyde
MH  - Kinetics
MH  - Mice
MH  - Mitomycin
MH  - Mitomycins/*pharmacology
MH  - RNA/metabolism
MH  - Staining and Labeling
MH  - Vincristine/*pharmacology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1177/39.1.1701186 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1991 Jan;39(1):15-21. doi: 10.1177/39.1.1701186.