PMID- 1701206
OWN - NLM
STAT- MEDLINE
DCOM- 19910115
LR  - 20220331
IS  - 0026-2862 (Print)
IS  - 0026-2862 (Linking)
VI  - 40
IP  - 2
DP  - 1990 Sep
TI  - Substance P stimulates neovascularization in vivo and proliferation of cultured 
      endothelial cells.
PG  - 264-78
AB  - We have investigated the possible effect of substance P (SP), a main mediator of 
      neurogenic inflammation, on the growth of capillary vessels in vivo, and on the 
      proliferation of cultured endothelial cells in vitro. Slow release preparations 
      of SP were implanted into the avascular cornea of New Zealand White rabbits and 
      vessel growth was monitored daily through a slit lamp stereomicroscope. SP (1-5 
      micrograms/pellet) induced a marked neovascularization. A selective NK-1 receptor 
      agonist [beta-Ala4, Sar9, Met(O2)11]-SP(4-11) also induced neovascularization. 
      The addition of SP to serum-free cultured endothelial cells, isolated from bovine 
      adrenals (BACE) and from human umbilical cord veins (HUVE), increased 
      proliferation of both cell lines in a concentration-dependent manner with maximal 
      activity at 10(-8) M (BACE) and 10(-10) M (HUVE). The selective NK-1 receptor 
      agonist induced a similar proliferative action on both cell lines, while the 
      selective NK-2 receptor agonist [beta-Ala8]-NKA(4-10) and the selective NK-3 
      receptor agonist [MePhe7]-NKB had no significant effect. Two different SP 
      antagonists [D-Pro2, D-Trp7,9]-SP and [D-Pro4, D-Trp7,9,Phe11]-SP (4-11) blocked 
      the response to SP. These findings indicate that SP can directly stimulate the 
      process of neovascularization, probably through induction of endothelial cell 
      proliferation. This hitherto unraveled activity of SP could play a key role in 
      the trophic action produced by activation of the efferent function of peripheral 
      endings of primary sensory neurons.
FAU - Ziche, M
AU  - Ziche M
AD  - Department of Preclinical and Clinical Pharmacology, Mario Aiazzi Mancini, 
      University of Florence, Italy.
FAU - Morbidelli, L
AU  - Morbidelli L
FAU - Pacini, M
AU  - Pacini M
FAU - Geppetti, P
AU  - Geppetti P
FAU - Alessandri, G
AU  - Alessandri G
FAU - Maggi, C A
AU  - Maggi CA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - 0 (Receptors, Neurotransmitter)
RN  - 0 (Receptors, Tachykinin)
RN  - 0 (Tachykinins)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Endothelium, Vascular/cytology/*drug effects/metabolism
MH  - *Neovascularization, Pathologic
MH  - Rabbits
MH  - Receptors, Neurotransmitter/drug effects/metabolism
MH  - Receptors, Tachykinin
MH  - Substance P/antagonists & inhibitors/*pharmacology
MH  - Tachykinins/metabolism
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
AID - 10.1016/0026-2862(90)90024-l [doi]
PST - ppublish
SO  - Microvasc Res. 1990 Sep;40(2):264-78. doi: 10.1016/0026-2862(90)90024-l.