PMID- 17013428
OWN - NLM
STAT- MEDLINE
DCOM- 20070103
LR  - 20061031
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 38
IP  - 10
DP  - 2006 Nov
TI  - Synergistic effects of injection of bone marrow cells into both portal vein and 
      bone marrow on tolerance induction in transplantation of allogeneic pancreatic 
      islets.
PG  - 657-64
AB  - We have established a new method for allogeneic pancreatic islet (PI) 
      transplantation: relatively low doses of irradiation followed by simultaneous 
      transplantation of PIs and bone marrow cells (BMCs) via the portal vein (PV). In 
      the present study, we have compared this method with intra-bone marrow (IBM)-bone 
      marrow transplantation (BMT), and with a combination of both methods. 
      Streptozotocin (STZ)-induced diabetic-recipient rats, Fischer 344 (F344, RT1A(l), 
      RT1B(l)), were irradiated 1 day before transplantation. PIs of Brown Norway rats 
      (BN, RT1A(n), RT1B(n)) were transplanted into the liver of the diabetic F344 rats 
      via the PV. BMCs from BN rats were injected into the recipients' bone marrow 
      (IBM), PV or intravenously (IV) or by a simultaneous combination of PV plus IBM 
      (PV+IBM). We compared graft survival among the groups of '9 Gy+IBM'(10/10 
      accepted), '9 Gy+PV'(7/10 accepted), '9 Gy+IV'(0/7 accepted), '9 Gy+PV+IBM'(8/8 
      accepted), '8.5 Gy+IBM'(4/9 accepted), '8.5 Gy+PV'(0/7 accepted), '8.5 Gy+IV'(0/7 
      accepted), '8.5 Gy+PV+IBM'(9/12 accepted), '8 Gy+IBM'(2/10 accepted) and '8 
      Gy+PV+IBM'(2/8 accepted). As we reported previously, PV-BMT is more effective in 
      inducing the acceptance of allogeneic PIs than IV-BMT. However, IBM-BMT requires 
      less pretreatment than PV-BMT. (PV+IBM)-BMT was found to be the most effective in 
      inducing the acceptance of allogeneic PIs. These results suggest that allogeneic 
      PI-transplantation in conjunction with (PV+IBM)-BMT could become a viable 
      strategy.
FAU - Ikebukuro, K
AU  - Ikebukuro K
AD  - First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Osaka 570-8506, Japan.
FAU - Adachi, Y
AU  - Adachi Y
FAU - Suzuki, Y
AU  - Suzuki Y
FAU - Iwasaki, M
AU  - Iwasaki M
FAU - Nakano, K
AU  - Nakano K
FAU - Koike, Y
AU  - Koike Y
FAU - Mukaide, H
AU  - Mukaide H
FAU - Yamada, Y
AU  - Yamada Y
FAU - Fujimoto, S
AU  - Fujimoto S
FAU - Seino, Y
AU  - Seino Y
FAU - Oyaizu, H
AU  - Oyaizu H
FAU - Shigematsu, A
AU  - Shigematsu A
FAU - Kiriyama, N
AU  - Kiriyama N
FAU - Hamada, Y
AU  - Hamada Y
FAU - Kamiyama, Y
AU  - Kamiyama Y
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061002
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Blood Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - Diabetes Mellitus, Experimental/blood/immunology/therapy
MH  - Female
MH  - Graft Survival
MH  - Immune Tolerance
MH  - Islets of Langerhans Transplantation/*methods/pathology/physiology
MH  - Male
MH  - Portal Vein
MH  - Rats
MH  - Rats, Inbred ACI
MH  - Rats, Inbred BN
MH  - Rats, Inbred F344
MH  - Transplantation, Homologous
EDAT- 2006/10/03 09:00
MHDA- 2007/01/04 09:00
CRDT- 2006/10/03 09:00
PHST- 2006/10/03 09:00 [pubmed]
PHST- 2007/01/04 09:00 [medline]
PHST- 2006/10/03 09:00 [entrez]
AID - 1705500 [pii]
AID - 10.1038/sj.bmt.1705500 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 2006 Nov;38(10):657-64. doi: 10.1038/sj.bmt.1705500. Epub 
      2006 Oct 2.