PMID- 17013928
OWN - NLM
STAT- MEDLINE
DCOM- 20070103
LR  - 20091119
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 66
IP  - 13
DP  - 2006 Nov
TI  - Brain-derived neurotrophic factor selectively regulates dendritogenesis of 
      parvalbumin-containing interneurons in the main olfactory bulb through the 
      PLCgamma pathway.
PG  - 1437-51
AB  - Molecular mechanisms of neurotrophin signaling on dendrite development and 
      dynamics are only partly understood. To address the role of brain-derived 
      neurotrophic factor (BDNF) in the morphogenesis of GABAergic neurons of the main 
      olfactory bulb, we analyzed mice lacking BDNF, mice carrying neurotrophin-3 (NT3) 
      in the place of BDNF, and TrkB signaling mutant mice with a receptor that can 
      activate phospholipase Cgamma (PLCgamma) but is unable to recruit the adaptors 
      Shc/Frs2. BDNF deletion yielded a compressed olfactory bulb with a significant 
      loss of parvalbumin (PV) immunoreactivity in GABAergic interneurons of the 
      external plexiform layer. Dendrite development of PV-positive interneurons was 
      selectively attenuated by BDNF since other Ca2+ -binding protein-containing 
      neuron populations appeared unaffected. The deficit in PV-positive neurons could 
      be rescued by the NT3/NT3 alleles. The degree of PV immunoreactivity was 
      dependent on BDNF and TrkB recruitment of the adaptor proteins Shc/Frs2. In 
      contrast, PLCgamma signaling from the TrkB receptor was sufficient for dendrite 
      growth in vivo and consistently, blocking PLCgamma prevented BDNF-dependent 
      dendrite development in vitro. Collectively, our results provide genetic evidence 
      that BDNF and TrkB signaling selectively regulate PV expression and dendrite 
      growth in a subset of neurochemically-defined GABAergic interneurons via 
      activation of the PLCgamma pathway.
CI  - Copyright 2006 Wiley Periodicals, Inc.
FAU - Berghuis, Paul
AU  - Berghuis P
AD  - Division of Molecular Neurobiology, Department of Medical Biochemistry and 
      Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden. 
      Patrik.Ernfors@ki.se
FAU - Agerman, Karin
AU  - Agerman K
FAU - Dobszay, Marton B
AU  - Dobszay MB
FAU - Minichiello, Liliana
AU  - Minichiello L
FAU - Harkany, Tibor
AU  - Harkany T
FAU - Ernfors, Patrik
AU  - Ernfors P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/deficiency/*pharmacology
MH  - Cells, Cultured
MH  - Dendrites/*drug effects/ultrastructure
MH  - Drug Interactions
MH  - Gene Expression Regulation, Developmental/drug effects/physiology
MH  - Immunohistochemistry/methods
MH  - *Interneurons/cytology/drug effects/metabolism
MH  - Membrane Potentials/drug effects/physiology/radiation effects
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Tissue Proteins/metabolism
MH  - Olfactory Bulb/*cytology
MH  - Parvalbumins/*metabolism
MH  - Patch-Clamp Techniques/methods
MH  - Phospholipase C gamma/*metabolism/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/genetics
MH  - Signal Transduction/physiology
MH  - Silver Staining/methods
EDAT- 2006/10/03 09:00
MHDA- 2007/01/04 09:00
CRDT- 2006/10/03 09:00
PHST- 2006/10/03 09:00 [pubmed]
PHST- 2007/01/04 09:00 [medline]
PHST- 2006/10/03 09:00 [entrez]
AID - 10.1002/neu.20319 [doi]
PST - ppublish
SO  - J Neurobiol. 2006 Nov;66(13):1437-51. doi: 10.1002/neu.20319.