PMID- 17014502
OWN - NLM
STAT- MEDLINE
DCOM- 20061122
LR  - 20061003
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 8
IP  - 11
DP  - 2006 Nov
TI  - Vertical distribution of nitrifying populations in bacterial biofilms from a 
      full-scale nitrifying trickling filter.
PG  - 2036-49
AB  - Cryosectioned biofilm from three depths (0.5, 3.0 and 6.0 m) in a full-scale 
      nitrifying trickling filter (NTF) were studied using fluorescence in situ 
      hybridization (FISH). A large number of sections were used to determine how the 
      biofilm thickness, structure and community composition varied with depth along 
      the ammonium concentration gradient in the NTF, and how the ammonia-oxidizing 
      bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were distributed vertically 
      within the biofilm. Both the biofilm thickness and relative biomass content of 
      the biofilm decreased with depth, along with structural differences such as void 
      size and surface roughness. Four AOB populations were found, with two 
      Nitrosomonas oligotropha populations dominating at all depths. A smaller 
      population of Nitrosomonas europaea was present only at 0.5 m, while a population 
      of Nitrosomonas communis increased with depth. The two N. oligotropha populations 
      showed different vertical distribution patterns within the biofilm, indicating 
      different ecophysiologies even though they belong to the same AOB lineage. All 
      NOB were identified as Nitrospira sp., and were generally more associated with 
      the biofilm base than the surface-associated dominating AOB population. 
      Additionally, a small population of anaerobic ammonia-oxidizers was found at 6.0 
      m, even though the biofilm was well aerated.
FAU - Lydmark, Par
AU  - Lydmark P
AD  - Department of Cell and Molecular Biology, Microbiology, Goteborg University, Box 
      462, SE-40530, Goteborg, Sweden. par.lydmark@gmm.gu.se
FAU - Lind, Magnus
AU  - Lind M
FAU - Sorensson, Fred
AU  - Sorensson F
FAU - Hermansson, Malte
AU  - Hermansson M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/metabolism
MH  - *Biofilms
MH  - Cryoultramicrotomy
MH  - Filtration/instrumentation
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrites/*metabolism
MH  - Nitrosomonas/cytology/*growth & development/metabolism
MH  - Oxidation-Reduction
MH  - Water Purification/methods
EDAT- 2006/10/04 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/10/04 09:00
PHST- 2006/10/04 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/04 09:00 [entrez]
AID - EMI1085 [pii]
AID - 10.1111/j.1462-2920.2006.01085.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2006 Nov;8(11):2036-49. doi: 10.1111/j.1462-2920.2006.01085.x.