PMID- 17014914
OWN - NLM
STAT- MEDLINE
DCOM- 20070205
LR  - 20180815
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 107
IP  - 2
DP  - 2006 Nov 15
TI  - IL-18 enhances the migration ability of murine melanoma cells through the 
      generation of ROI and the MAPK pathway.
PG  - 125-30
AB  - Interleukin-18 (IL-18) has multiple effects on various cells that are involved in 
      immune escape of murine melanoma cells and in the inflammatory responses. This 
      study investigated the effect of IL-18 on the ability of murine melanoma cells to 
      migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 
      cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able 
      to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 
      treatment improved the ability of the B16F10/IL-18 antisense transfectant cells 
      to migrate, indicating that IL-18 enhanced the migration ability of melanoma 
      cells. To determine the signaling mechanisms involved in IL-18-enhanced 
      migration, we measured the ROI levels. It was found that the ROI levels were 
      increased by IL-18, and an antioxidant, N-acetyl-l-cystein (NAC), blocked the 
      effect of IL-18 on migration, suggesting the involvement of ROI in the signal 
      transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was 
      also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was 
      markedly increased by treating with exogenous IL-18 at 20 min. These results 
      suggest that IL-18 enhances the ability of melanoma cells to migrate via the 
      generation of ROI and the MAPK pathway.
FAU - Jung, Min Kyung
AU  - Jung MK
AD  - Department of Life Science, Sookmyung Women's University, Chungpa-Dong 2-Ka, 
      Yongsan-ku, Seoul 140-742, Republic of Korea.
FAU - Song, Hyun Keun
AU  - Song HK
FAU - Kim, Kyung-Eun
AU  - Kim KE
FAU - Hur, Dae Young
AU  - Hur DY
FAU - Kim, Taesung
AU  - Kim T
FAU - Bang, Saic
AU  - Bang S
FAU - Park, Hyunjeong
AU  - Park H
FAU - Cho, Dae Ho
AU  - Cho DH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060911
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Interleukin-18)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Animals
MH  - *Cell Movement/drug effects
MH  - Flavonoids/pharmacology
MH  - Free Radical Scavengers/pharmacology
MH  - Interleukin-18/genetics/pharmacology/*physiology
MH  - Melanoma, Experimental/immunology/*pathology
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism
MH  - Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/metabolism
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Phosphorylation
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Reactive Oxygen Species/analysis/antagonists & inhibitors/*metabolism
MH  - Transfection
EDAT- 2006/10/04 09:00
MHDA- 2007/02/06 09:00
CRDT- 2006/10/04 09:00
PHST- 2006/05/11 00:00 [received]
PHST- 2006/08/14 00:00 [revised]
PHST- 2006/08/20 00:00 [accepted]
PHST- 2006/10/04 09:00 [pubmed]
PHST- 2007/02/06 09:00 [medline]
PHST- 2006/10/04 09:00 [entrez]
AID - S0165-2478(06)00207-0 [pii]
AID - 10.1016/j.imlet.2006.08.004 [doi]
PST - ppublish
SO  - Immunol Lett. 2006 Nov 15;107(2):125-30. doi: 10.1016/j.imlet.2006.08.004. Epub 
      2006 Sep 11.