PMID- 17015474
OWN - NLM
STAT- MEDLINE
DCOM- 20061113
LR  - 20220225
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 26
IP  - 20
DP  - 2006 Oct
TI  - Differentiation-induced cleavage of Cutl1/CDP generates a novel dominant-negative 
      isoform that regulates mammary gene expression.
PG  - 7466-78
AB  - Cutl1/CCAAT displacement protein (CDP) is a transcriptional repressor of mouse 
      mammary tumor virus (MMTV), a betaretrovirus that is a paradigm for 
      mammary-specific gene regulation. Virgin mammary glands have high levels of 
      full-length CDP (200 kDa) that binds to negative regulatory elements (NREs) to 
      repress MMTV transcription. During late pregnancy, full-length CDP levels 
      decline, and a 150-kDa form of CDP (CDP150) appears concomitantly with a decline 
      in DNA-binding activity for the MMTV NREs and an increase in viral transcripts. 
      Developmental regulation of CDP was recapitulated in the normal mammary 
      epithelial line, SCp2. Western blotting of tissue and SCp2 nuclear extracts 
      confirmed that CDP150 lacks the C terminus. Transfection of tagged full-length 
      and mutant cDNAs into SCp2 cells and use of a cysteine protease inhibitor 
      demonstrated that CDP is proteolytically processed within the homeodomain to 
      remove the C terminus during differentiation. Mixing of virgin and lactating 
      mammary extracts or transfection of mutant CDP cDNAs missing the homeodomain into 
      cells containing full-length CDP also abrogated NRE binding. Loss of DNA binding 
      correlated with increased expression of MMTV and other mammary-specific genes, 
      indicating that CDP150 is a developmentally induced dominant-negative protein. 
      Thus, a novel posttranslational process controls Cutl1/CDP activity and gene 
      expression in the mammary gland.
FAU - Maitra, Urmila
AU  - Maitra U
AD  - Section of Molecular Genetics and Microbiology, The University of Texas at 
      Austin, 24th and Speedway, ESB 226, Austin, TX 78712-0162, USA.
FAU - Seo, Jin
AU  - Seo J
FAU - Lozano, Mary M
AU  - Lozano MM
FAU - Dudley, Jaquelin P
AU  - Dudley JP
LA  - eng
GR  - R01CA34780/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20060805
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Caseins)
RN  - 0 (Cux1 protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Repressor Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
SB  - IM
MH  - Animals
MH  - Caseins/metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Cysteine Endopeptidases/metabolism
MH  - DNA/metabolism
MH  - Female
MH  - Gene Deletion
MH  - *Gene Expression
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Mammary Glands, Animal/*cytology/*metabolism
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Pregnancy
MH  - Protein Binding
MH  - Protein Isoforms/genetics/metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - Response Elements/genetics
MH  - Transcription, Genetic/genetics
PMC - PMC1636867
EDAT- 2006/10/04 09:00
MHDA- 2006/11/14 09:00
PMCR- 2007/02/01
CRDT- 2006/10/04 09:00
PHST- 2006/10/04 09:00 [pubmed]
PHST- 2006/11/14 09:00 [medline]
PHST- 2006/10/04 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - MCB.01083-06 [pii]
AID - 1083-06 [pii]
AID - 10.1128/MCB.01083-06 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2006 Oct;26(20):7466-78. doi: 10.1128/MCB.01083-06. Epub 2006 Aug 
      5.