PMID- 17017159
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 4
DP  - 2006 Jul
TI  - [Optimization of high performance liquid chromatographic method for analysis of 
      kudzu root crude extract].
PG  - 354-8
AB  - Kudzu root is an important traditional Chinese herb. Its crude extract has been 
      used in the treatment of hypertension and angina pectoris in China. Several 
      parameters for high performance liquid chromatography (HPLC) analytical method 
      for kudzu root extract, including HPLC model, mobile phase, additive and gradient 
      conditions have been optimized. The HPLC retention parameters a and c, and the 
      peak shape parameters sigma and tau of 25 chromatographic peaks were obtained 
      accurately and rapidly using five linear gradients and were calculated using 
      CSASS software. The CSASS software was then used to simulate the gradient 
      conditions in the experiments, and the optimized condition was obtained. The 
      comparison of the simulated and real chromatogram profiles showed that the 
      potential of simulation using the CSSASS software as compared to that of the real 
      experimental conditions was precise. Reversed-phase HPLC model and the mobile 
      phase of (A) acetonitrile containing 0.1% (v/v) acetic acid and (B) water 
      containing 0.1% (v/v) acetic acid were used. The optimized gradient profile 
      showed a linear increase as follows: from 5% A to 35% A at 0 - 33 min; from 35% A 
      to 100% A at 33 - 50 min, and then the HPLC system was held for 5 min. The 
      reproducibility and precision of the method were investigated. The relative 
      standard deviations (RSDs) of retention time, peak height, and peak area were 
      less than 0.25%, 11%, and 4.9%, respectively, which showed that the method was 
      stable, reliable, and reproducible.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, 
      China. zhangyanzy@dicp.ac.cn
FAU - Xue, Xingya
AU  - Xue X
FAU - Xu, Qing
AU  - Xu Q
FAU - Zhang, Feifang
AU  - Zhang F
FAU - Liang, Xinmiao
AU  - Liang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Flowers/*chemistry
MH  - Plant Extracts/chemistry
MH  - Plant Roots/*chemistry
MH  - Pueraria/*chemistry
MH  - Spectrophotometry, Ultraviolet/methods
EDAT- 2006/10/05 09:00
MHDA- 2010/07/08 06:00
CRDT- 2006/10/05 09:00
PHST- 2006/10/05 09:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2006/10/05 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Jul;24(4):354-8.