PMID- 17017160
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 4
DP  - 2006 Jul
TI  - [Simultaneous determination of 17 underivatized amino acids in donkey-hide glue 
      by reversed-phase high performance liquid chromatography-evaporative 
      light-scattering detection].
PG  - 359-62
AB  - An analytical method to determine 17 underivatized amino acids in donkey-hide 
      glue was established with reversed-phase high performance liquid chromatography 
      (HPLC) coupled with evaporative light-scattering detection (ELSD). A Prevail C18 
      column was used with the mobile phase of acetonitrile-0.7% trifluoroacetic acid 
      containing 5.0 mmol/L heptafluorobutyric acid. Under the condition of solvent 
      gradient elution, the temperature of drift tube was 115 degrees C and the gas 
      flow rate was 2.5 L/min. The 17 amino acids were separated within 25 min. The 
      good linearities between the logarithm of peak area and logarithm of mass 
      concentration of amino acids were obtained in a range of mass concentrations from 
      0.073 g/L to 2.327 g/L. The recoveries of 17 amino acids were 93.5% - 104.8% with 
      the relative standard deviations (RSDs) of 0.58% - 2.88%. The lowest detection 
      limits of amino acids were from 18.2 mg/L to 54.6 mg/L with 3 times the signal to 
      noise ratio. This HPLC-ELSD method is rapid, simple and accurate. It can be used 
      for the direct determination of 17 underivatized amino acids in donkey-hide glue. 
      It also serves as a good reference for the determination of amino acids in other 
      fields, such as pharmaceutical analysis.
FAU - Yan, Dan
AU  - Yan D
AD  - Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing 
      100039, China. yd277@126.com
FAU - Han, Yumei
AU  - Han Y
FAU - Dong, Xiaoping
AU  - Dong X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Light
MH  - Limit of Detection
MH  - Medicine, Chinese Traditional
MH  - Plant Stems
EDAT- 2006/10/05 09:00
MHDA- 2010/07/08 06:00
CRDT- 2006/10/05 09:00
PHST- 2006/10/05 09:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2006/10/05 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Jul;24(4):359-62.