PMID- 17019695 OWN - NLM STAT- MEDLINE DCOM- 20070206 LR - 20061221 IS - 1059-910X (Print) IS - 1059-910X (Linking) VI - 70 IP - 1 DP - 2007 Jan TI - Electron microscopic study of novel threadlike structures on the surfaces of mammalian organs. PG - 34-43 AB - The ultrastructures of novel threadlike structures (NTSs) and corpuscles on the surfaces of internal organs of rats were investigated using electron microscopy. The samples were studied in situ by using a stereomicroscope and were taken for further morphological analysis. Scanning electron microscope (SEM) images revealed a bundle structure of threadlike tissue, which was composed of several 10-micro m-thick subducts. The surfaces of the corpuscles were rather coarse and fenestrated. The corpuscles had cucumber-like shapes with an average length of about 2 mm and a thickness of about 400 micro m. Transmission electron microscope (TEM) images disclosed disordered collagen fibers, which formed the extracellular matrix of the threadlike tissue, and immune-function cells, like macrophages, mast cells, and eosinophils. Sinuses of various diameters, which were thought to be cross-sections of the lumens of the subducts, were observed in the TEM, cryo-SEM and focused-ion-beam SEM images. These SEM images were obtained for the first time to reveal the detailed structure of the NTSs that were only recently discovered. FAU - Lee, Byung-Cheon AU - Lee BC AD - Biomedical Physics Laboratory, FPRD, School of Physics and Astronomy, Seoul National University, Seoul, Korea. FAU - Yoo, Jung Sun AU - Yoo JS FAU - Ogay, Vyacheslav AU - Ogay V FAU - Kim, Ki Woo AU - Kim KW FAU - Dobberstein, Harald AU - Dobberstein H FAU - Soh, Kwang-Sup AU - Soh KS FAU - Chang, Byung-Soo AU - Chang BS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Microsc Res Tech JT - Microscopy research and technique JID - 9203012 RN - 0 (Fibrillar Collagens) SB - IM MH - Acupuncture Points MH - Animals MH - Cryoelectron Microscopy/*methods MH - Eosinophils/ultrastructure MH - Fibrillar Collagens/ultrastructure MH - Intestine, Large/anatomy & histology/*ultrastructure MH - Intestine, Small/anatomy & histology/*ultrastructure MH - Macrophages/ultrastructure MH - Mast Cells/ultrastructure MH - Microscopy, Electron, Scanning MH - Microscopy, Electron, Transmission/*methods MH - Rabbits MH - Rats MH - Rats, Wistar EDAT- 2006/10/05 09:00 MHDA- 2007/02/07 09:00 CRDT- 2006/10/05 09:00 PHST- 2006/10/05 09:00 [pubmed] PHST- 2007/02/07 09:00 [medline] PHST- 2006/10/05 09:00 [entrez] AID - 10.1002/jemt.20383 [doi] PST - ppublish SO - Microsc Res Tech. 2007 Jan;70(1):34-43. doi: 10.1002/jemt.20383.