PMID- 17021106
OWN - NLM
STAT- MEDLINE
DCOM- 20061121
LR  - 20240412
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 44
IP  - 10
DP  - 2006 Oct
TI  - Evaluation of peptide nucleic acid-fluorescence in situ hybridization for 
      identification of clinically relevant mycobacteria in clinical specimens and 
      tissue sections.
PG  - 3760-7
AB  - With fluorescently labeled PNA (peptide nucleic acid) probes targeting 16S rRNA, 
      we established a 3-h fluorescence in situ hybridization (FISH) procedure for 
      specific visualization of members of the Mycobacterium tuberculosis complex, M. 
      leprae, M. avium, and M. kansasii. Probe specificity was tested against a panel 
      of 25 Mycobacterium spp. and 10 gram-positive organisms. After validation, probes 
      were used to identify 52 mycobacterial culture isolates. Results were compared to 
      conventional genotypic identification with amplification-based methods. All 
      isolates (M. tuberculosis complex, n = 24; M. avium, n = 7; M. kansasii, n = 1) 
      were correctly identified by FISH. In addition, the technique was used 
      successfully for visualization of mycobacteria in biopsies from infected humans 
      or animals. In conclusion, PNA-FISH is a fast and accurate tool for 
      species-specific identification of culture-grown mycobacteria and for direct 
      visualization of these organisms in tissue sections. It may be used successfully 
      for both research and clinical microbiology.
FAU - Lefmann, Michael
AU  - Lefmann M
AD  - Institut fur Mikrobiologie und Hygiene, Charite-Universitatsmedizin Berlin, 
      Dorotheenstr. 96, 10117 Berlin, Germany. annette.moter@charite.de.
FAU - Schweickert, Birgitta
AU  - Schweickert B
FAU - Buchholz, Petra
AU  - Buchholz P
FAU - Gobel, Ulf B
AU  - Gobel UB
FAU - Ulrichs, Timo
AU  - Ulrichs T
FAU - Seiler, Peter
AU  - Seiler P
FAU - Theegarten, Dirk
AU  - Theegarten D
FAU - Moter, Annette
AU  - Moter A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mycobacterium/*classification/isolation & purification
MH  - Mycobacterium Infections/*diagnosis/microbiology
MH  - Nucleic Acid Probes
MH  - *Peptide Nucleic Acids
MH  - Sensitivity and Specificity
PMC - PMC1594750
EDAT- 2006/10/06 09:00
MHDA- 2006/12/09 09:00
PMCR- 2007/02/01
CRDT- 2006/10/06 09:00
PHST- 2006/10/06 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/06 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 44/10/3760 [pii]
AID - 1435-06 [pii]
AID - 10.1128/JCM.01435-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2006 Oct;44(10):3760-7. doi: 10.1128/JCM.01435-06.