PMID- 17021875
OWN - NLM
STAT- MEDLINE
DCOM- 20070529
LR  - 20220410
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 73
IP  - 6
DP  - 2007 Jan
TI  - Agrobacterium-mediated transformation (AMT) of Trichoderma reesei as an efficient 
      tool for random insertional mutagenesis.
PG  - 1348-54
AB  - Filamentous fungus Trichoderma reesei QM9414 was successfully transformed with 
      Agrobacterium tumefaciens AGL-1 for random integration of transforming DNA 
      (T-DNA). Co-cultivation of T. reesei conidia or protoplasts with A. tumefaciens 
      in the presence of acetosyringone resulted in the formation of hygromycin 
      B-resistant fungal colonies with high transformation frequency. Nine randomly 
      selected resistant clones were proved to be stable through mitotic cell division. 
      The integration of the hph gene into T. reesei genome was determined by PCR and 
      dot blot analysis. Transgenic T. reesei strains were analyzed using TAIL-PCR for 
      their T-DNA contents. The results showed that T-DNA inserts occurred evidently by 
      fusing DNA at T-DNA borders via random recombination, which suggests that 
      Agrobacterium-mediated transformation is a potentially powerful tool towards 
      tagged mutagenesis and gene transfer technology for T. reesei.
FAU - Zhong, Yao Hua
AU  - Zhong YH
AD  - State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, 
      Shandong, People's Republic of China.
FAU - Wang, Xiao Li
AU  - Wang XL
FAU - Wang, Tian Hong
AU  - Wang TH
FAU - Jiang, Qiao
AU  - Jiang Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061005
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (DNA, Fungal)
RN  - 3XQ2233B0B (Hygromycin B)
SB  - IM
MH  - Agrobacterium tumefaciens/*genetics
MH  - Base Sequence
MH  - DNA, Fungal/chemistry/genetics
MH  - Genome, Fungal
MH  - Hygromycin B/pharmacology
MH  - Mutagenesis, Insertional/*methods
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transformation, Genetic/*genetics
MH  - Trichoderma/drug effects/*genetics
EDAT- 2006/10/06 09:00
MHDA- 2007/05/30 09:00
CRDT- 2006/10/06 09:00
PHST- 2006/06/12 00:00 [received]
PHST- 2006/08/07 00:00 [accepted]
PHST- 2006/07/31 00:00 [revised]
PHST- 2006/10/06 09:00 [pubmed]
PHST- 2007/05/30 09:00 [medline]
PHST- 2006/10/06 09:00 [entrez]
AID - 10.1007/s00253-006-0603-3 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2007 Jan;73(6):1348-54. doi: 
      10.1007/s00253-006-0603-3. Epub 2006 Oct 5.