PMID- 1702200
OWN - NLM
STAT- MEDLINE
DCOM- 19910201
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 18
IP  - 23
DP  - 1990 Dec 11
TI  - Activating-transcription-factor (ATF) regulates human 7S L RNA transcription by 
      RNA polymerase III in vivo and in vitro.
PG  - 6779-84
AB  - The gene-external part of the human 7S L promoter was analyzed by transcription 
      in vitro and in vivo. Compared to the wild type promoter (-178), a -66 5'deletion 
      mutant revealed full activity in vitro but was inefficiently transcribed in vivo. 
      Further deletion to -37 reduced template activity to 50% in vitro and to basal 
      level expression in vivo (below 5%). A DNase I footprint observed around position 
      -50 protected an ATF-like binding site ('TGACGT'). With respect to 7S L 
      transcription regulation, the functionality of this ATF-like binding site was 
      confirmed in competition experiments and by mutation analysis. Furthermore, S100 
      extracts of cells pretreated with forskolin in vivo to induce the cAMP system, 
      revealed significantly increased transcription of 7S L RNA in vitro, with no 
      effect on a 7S K RNA gene, lacking such an ATF binding site. Thus, the 7S L RNA 
      gene too is controlled by a regulatory element originally defined in class II 
      promoters and represents another rare example where a specific type of 
      transcription regulation in vivo can be mimicked with cell-free extracts in 
      vitro.
FAU - Bredow, S
AU  - Bredow S
AD  - Department of Biochemistry, Faculty of Chemistry, Ruhr-University, Bochum, FRG.
FAU - Surig, D
AU  - Surig D
FAU - Muller, J
AU  - Muller J
FAU - Kleinert, H
AU  - Kleinert H
FAU - Benecke, B J
AU  - Benecke BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Activating Transcription Factors)
RN  - 0 (Blood Proteins)
RN  - 0 (Transcription Factors)
RN  - 1F7A44V6OU (Colforsin)
RN  - 63231-63-0 (RNA)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
GS  - 7S L
MH  - Activating Transcription Factors
MH  - Base Sequence
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Blood Proteins/*metabolism
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - DNA Mutational Analysis
MH  - *Gene Expression Regulation
MH  - Genes
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - RNA/*genetics
MH  - RNA Polymerase III/*metabolism
MH  - Transcription Factors/*metabolism
MH  - *Transcription, Genetic
MH  - Transfection
PMC - PMC332731
EDAT- 1990/12/11 00:00
MHDA- 1990/12/11 00:01
CRDT- 1990/12/11 00:00
PHST- 1990/12/11 00:00 [pubmed]
PHST- 1990/12/11 00:01 [medline]
PHST- 1990/12/11 00:00 [entrez]
AID - 10.1093/nar/18.23.6779 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1990 Dec 11;18(23):6779-84. doi: 10.1093/nar/18.23.6779.