PMID- 1702516
OWN - NLM
STAT- MEDLINE
DCOM- 19910214
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 245
IP  - 4
DP  - 1990 Dec
TI  - The micronucleus assay with mouse peripheral blood reticulocytes using acridine 
      orange-coated slides.
PG  - 245-9
AB  - Ultra-vital staining with acridine orange (AO) is introduced into the 
      micronucleus assay with mouse peripheral blood cells. Peripheral blood was 
      stained vitally by dropping whole blood on an AO-coated slide and covering the 
      sample with a coverslip. With this method, reticulocytes are identified easily by 
      their red fluorescing reticulum structure. The distinction between young and 
      mature erythrocytes was clearer and less subjective than the distinction between 
      polychromatic and normochromatic erythrocytes by Giemsa staining or by 
      conventional AO fluorescent staining. Although the induction of micronucleated 
      peripheral reticulocytes (MNRETs) was delayed by about 12 h compared to that of 
      micronucleated polychromatic erythrocytes (MNPCEs) in the bone marrow, the 
      frequencies of MNRETs and MNPCEs were almost identical at each optimal sampling 
      time. It is concluded that bone marrow cells can be replaced by peripheral blood 
      as material for the micronucleus assay.
FAU - Hayashi, M
AU  - Hayashi M
AD  - Division of Genetics and Mutagenesis, Biological Safety Research Center, National 
      Institute of Hygienic Sciences, Tokyo, Japan.
FAU - Morita, T
AU  - Morita T
FAU - Kodama, Y
AU  - Kodama Y
FAU - Sofuni, T
AU  - Sofuni T
FAU - Ishidate, M Jr
AU  - Ishidate M Jr
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Alkylating Agents)
RN  - 0 (Mitomycins)
RN  - 50SG953SK6 (Mitomycin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Alkylating Agents/toxicity
MH  - Animals
MH  - Bone Marrow Cells
MH  - Male
MH  - Mice
MH  - Micronuclei, Chromosome-Defective/*ultrastructure
MH  - Micronucleus Tests/*methods
MH  - Microscopy, Fluorescence
MH  - Mitomycin
MH  - Mitomycins/toxicity
MH  - Reticulocytes/*ultrastructure
MH  - Staining and Labeling
EDAT- 1990/12/11 19:15
MHDA- 2001/03/28 10:01
CRDT- 1990/12/11 19:15
PHST- 1990/12/11 19:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1990/12/11 19:15 [entrez]
AID - 0165-7992(90)90153-B [pii]
AID - 10.1016/0165-7992(90)90153-b [doi]
PST - ppublish
SO  - Mutat Res. 1990 Dec;245(4):245-9. doi: 10.1016/0165-7992(90)90153-b.