PMID- 17028863
OWN - NLM
STAT- MEDLINE
DCOM- 20070917
LR  - 20220716
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 245
IP  - 5
DP  - 2007 May
TI  - Matrix metalloproteinases and their inhibitors in the chamber angle of normal 
      eyes and patients with primary open-angle glaucoma and exfoliation glaucoma.
PG  - 697-704
AB  - BACKGROUND: In glaucoma, extensive pathological changes occur in the trabecular 
      meshwork (TM) and juxtacanalicular tissue of the chamber angle. Aqueous humor 
      drainage is disturbed due to the accumulation of extracellular matrix (ECM) 
      material in the outflow system. Matrix metalloproteinases (MMPs) remodel ECM 
      material and, thus, they may have a role in regulating outflow facility and 
      intraocular pressure (IOP). This study examined the expression of MMPs and tissue 
      inhibitors of MMPs (TIMPs) in the chamber angle of normal eyes and in primary 
      open-angle glaucoma (POAG) and in exfoliation glaucoma (ExG). METHODS: TM tissues 
      were isolated from healthy donor eyes for corneal transplantation. Specimens of 
      the inner wall of Schlemm's canal and the juxtacanalicular tissue were collected 
      from patients with POAG or ExG during deep sclerectomy operation. Monoclonal 
      antibodies against MMPs (MMP-1, -2, -3, and -9) and antibodies against TIMPs 
      (TIMP-1, -2, and -3) were used for immunohistochemical staining. RESULTS: 
      Immunoreactivity for MMP-2, TIMP-2, or TIMP-3 was observed in human normal TM and 
      in the inner wall of Schlemm's canal. In general, immunoreactions for all of the 
      tested MMPs were more intense in POAG samples than in ExG samples or in the 
      control group. The only exception was the MMP-2 level, which was the highest in 
      the control group. The staining intensity of MMP-1 or MMP-3 was significantly 
      higher in POAG when compared to ExG. TIMP-1 was significantly increased in POAG 
      compared with ExG and there were no marked differences in the levels of TIMP-2 or 
      TIMP-3 between POAG and ExG. The ratios of MMP-1/TIMP-1 and MMP(1+2+3+9) and 
      TIMP(1+2+3) were significantly higher in samples from POAG compared to those of 
      ExG. CONCLUSIONS: Our results reveal an expression imbalance between MMPs and 
      their endogenous tissue inhibitors in tissue samples from patients with POAG and 
      ExG. Differences in immunohistochemical reactions reflect discrete local 
      pathogenic mechanisms involved in POAG and ExG. With respect to the proposed role 
      of MMPs in the remodeling of ECM material, this may point to a weaker reactivity 
      to the accumulation of ECM material in TM in ExG than POAG eyes.
FAU - Ronkko, Seppo
AU  - Ronkko S
AD  - Department of Ophthalmology, University of Kuopio, 70211, Kuopio, Finland. 
      Seppo.Ronkko@uku.fi
FAU - Rekonen, Petri
AU  - Rekonen P
FAU - Kaarniranta, Kai
AU  - Kaarniranta K
FAU - Puustjarvi, Tuomo
AU  - Puustjarvi T
FAU - Terasvirta, Markku
AU  - Terasvirta M
FAU - Uusitalo, Hannu
AU  - Uusitalo H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061007
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Anterior Chamber/*enzymology
MH  - Exfoliation Syndrome/*enzymology
MH  - Female
MH  - Glaucoma, Open-Angle/*enzymology
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Middle Aged
MH  - Tissue Inhibitor of Metalloproteinases/*metabolism
MH  - Trabecular Meshwork/*enzymology
EDAT- 2006/10/10 09:00
MHDA- 2007/09/18 09:00
CRDT- 2006/10/10 09:00
PHST- 2006/04/07 00:00 [received]
PHST- 2006/08/07 00:00 [accepted]
PHST- 2006/06/22 00:00 [revised]
PHST- 2006/10/10 09:00 [pubmed]
PHST- 2007/09/18 09:00 [medline]
PHST- 2006/10/10 09:00 [entrez]
AID - 10.1007/s00417-006-0440-1 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2007 May;245(5):697-704. doi: 
      10.1007/s00417-006-0440-1. Epub 2006 Oct 7.