PMID- 17029981
OWN - NLM
STAT- MEDLINE
DCOM- 20070302
LR  - 20220310
IS  - 1044-7431 (Print)
IS  - 1095-9327 (Electronic)
IS  - 1044-7431 (Linking)
VI  - 33
IP  - 4
DP  - 2006 Dec
TI  - BDNF up-regulates alpha7 nicotinic acetylcholine receptor levels on 
      subpopulations of hippocampal interneurons.
PG  - 381-8
AB  - In the hippocampus, brain-derived neurotrophic factor (BDNF) regulates a number 
      of synaptic components. Among these are nicotinic acetylcholine receptors 
      containing alpha7 subunits (alpha7-nAChRs), which are interesting because of 
      their relative abundance in the hippocampus and their high relative calcium 
      permeability. We show here that BDNF elevates surface and intracellular pools of 
      alpha7-nAChRs on cultured hippocampal neurons and that glutamatergic activity is 
      both necessary and sufficient for the effect. Blocking transmission through NMDA 
      receptors with APV blocked the BDNF effect; increasing spontaneous excitatory 
      activity with the GABA(A) receptor antagonist bicuculline replicated the BDNF 
      effect. BDNF antibodies blocked the BDNF-mediated increase but not the 
      bicuculline one, consistent with enhanced glutamatergic activity acting 
      downstream from BDNF. Increased alpha7-nAChR clusters were most prominent on 
      interneuron subtypes known to directly innervate excitatory neurons. The results 
      suggest that BDNF, acting through glutamatergic transmission, can modulate 
      hippocampal output in part by controlling alpha7-nAChR levels.
FAU - Massey, Kerri A
AU  - Massey KA
AD  - Neurobiology Section, Division of Biological Sciences, 0357, University of 
      California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0357, USA. 
      kamassey@ucsd.edu
FAU - Zago, Wagner M
AU  - Zago WM
FAU - Berg, Darwin K
AU  - Berg DK
LA  - eng
GR  - R01 NS012601/NS/NINDS NIH HHS/United States
GR  - R01 NS035469/NS/NINDS NIH HHS/United States
GR  - NS12601/NS/NINDS NIH HHS/United States
GR  - NS35469/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061009
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Chrna7 protein, rat)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Drug Interactions
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glutamate Decarboxylase/metabolism
MH  - Hippocampus/*cytology
MH  - Immunohistochemistry/methods
MH  - Interneurons/*drug effects
MH  - Protein Transport/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Nicotinic/genetics/*metabolism
MH  - Time Factors
MH  - Up-Regulation/*drug effects
MH  - Valine/analogs & derivatives/pharmacology
MH  - alpha7 Nicotinic Acetylcholine Receptor
PMC - PMC1826088
MID - NIHMS14826
EDAT- 2006/10/13 09:00
MHDA- 2007/03/03 09:00
PMCR- 2008/02/01
CRDT- 2006/10/13 09:00
PHST- 2006/06/20 00:00 [received]
PHST- 2006/08/04 00:00 [revised]
PHST- 2006/08/30 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - S1044-7431(06)00192-8 [pii]
AID - 10.1016/j.mcn.2006.08.011 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2006 Dec;33(4):381-8. doi: 10.1016/j.mcn.2006.08.011. Epub 
      2006 Oct 9.