PMID- 17030251
OWN - NLM
STAT- MEDLINE
DCOM- 20061204
LR  - 20131121
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 118
IP  - 4
DP  - 2006 Oct
TI  - Potential involvement of dendritic cells in delayed-type hypersensitivity 
      reactions to beta-lactams.
PG  - 949-56
AB  - BACKGROUND: Although the involvement of T cells in delayed reactions to drugs has 
      been studied, little is known about the interaction between the drug and the 
      antigen-presenting cells. Dendritic cells (DCs) are professional 
      antigen-presenting cells essential for initiating T-cell responses. Their ability 
      is regulated in a process known as maturation, by which they modulate the 
      effector immune response. OBJECTIVES: We studied the role of DCs in subjects who 
      had a delayed-type hypersensitivity reaction to amoxicillin to assess the effect 
      on the pattern of maturation and determine the capacity of DCs to activate T 
      lymphocytes. METHODS: We examined the consequences of the interaction between 
      monocyte-derived DCs, lymphocytes, and amoxicillin by means of phenotypic and 
      functional studies, including endocytosis, proliferation, and cytokine 
      production. RESULTS: Amoxicillin drove DCs from hypersensitive subjects to a 
      phenotypic and functional semimature status, inducing a T-cell proliferation 
      response. CONCLUSIONS: In delayed reactions to amoxicillin, DCs play a relevant 
      role in inducing the T-cell responses. These results are useful not only to 
      understand the mechanism but potentially as a possible approach to diagnosis. 
      CLINICAL IMPLICATIONS: A better understanding of T-cell and DC involvement in 
      delayed-type hypersensitivity reactions is needed. This in vitro assay might 
      provide clues to the diagnostic evaluation of patients allergic to penicillins.
FAU - Rodriguez-Pena, Rebeca
AU  - Rodriguez-Pena R
AD  - Research Laboratory Carlos Haya Hospital-Fundacion IMABIS, Spain. 
      rebeca.rodriguez.exts@juntadeandalucia.es
FAU - Lopez, Soledad
AU  - Lopez S
FAU - Mayorga, Cristobalina
AU  - Mayorga C
FAU - Antunez, Cristina
AU  - Antunez C
FAU - Fernandez, Tahia D
AU  - Fernandez TD
FAU - Torres, Maria J
AU  - Torres MJ
FAU - Blanca, Miguel
AU  - Blanca M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060901
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (beta-Lactams)
RN  - 804826J2HU (Amoxicillin)
SB  - IM
MH  - Adult
MH  - Amoxicillin/adverse effects/immunology
MH  - Antigen Presentation/immunology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Drug Hypersensitivity/*immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity, Delayed/*chemically induced
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Subsets/immunology
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes/immunology
MH  - beta-Lactams/*adverse effects/immunology
EDAT- 2006/10/13 09:00
MHDA- 2006/12/09 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/04/19 00:00 [received]
PHST- 2006/07/04 00:00 [revised]
PHST- 2006/07/07 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - S0091-6749(06)01515-6 [pii]
AID - 10.1016/j.jaci.2006.07.013 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2006 Oct;118(4):949-56. doi: 10.1016/j.jaci.2006.07.013. 
      Epub 2006 Sep 1.