PMID- 17030875
OWN - NLM
STAT- MEDLINE
DCOM- 20061114
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 11
DP  - 2006 Nov
TI  - Identification of an HLA-A*0201-restricted cytotoxic T-lymphocyte epitope in 
      rotavirus VP6 protein.
PG  - 3393-3396
LID - 10.1099/vir.0.82031-0 [doi]
AB  - The function of cytotoxic T lymphocytes (CTLs) in rotavirus (RV) infection in 
      humans is poorly understood. To date, no RV-specific human leukocyte antigen 
      (HLA) class I-restricted T-cell epitopes have been described. In this study, four 
      peptides derived from human RV Wa strain VP6 protein were predicted by computer 
      algorithms and verified by an HLA*0201-binding assay. Two peptides with high 
      affinity for HLA-A*0201 molecules were further assessed. The CTLs induced in 
      vitro by P340-348 (TLLANVTAV)-loaded autologous dendritic cells from peripheral 
      blood lymphocytes of HLA-A*0201-matched healthy donors released gamma interferon 
      specifically upon stimulation with P340-348-loaded T2 cells. The CTLs lysed both 
      P340-348-loaded T2 cells and human RV Wa strain-infected HLA-A*0201(+) Caco-2 
      cells in an antigen-specific and HLA-A*0201-restricted manner. At the same time, 
      P340-348 was shown to be immunogenic in vivo in HLA-A*0201/Kb transgenic mice. It 
      is proposed that P340-348 is an HLA-A*0201-restricted CTL epitope.
FAU - Wei, Jing
AU  - Wei J
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Li, Jin-Tao
AU  - Li JT
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Zhang, Xiao-Ping
AU  - Zhang XP
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Tang, Yan
AU  - Tang Y
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Wang, Jing-Xue
AU  - Wang JX
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Zhang, Bei
AU  - Zhang B
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
FAU - Wu, Yu-Zhang
AU  - Wu YZ
AD  - Institute of Immunology, PLA, The Third Military Medical University, Chongqing 
      400038, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, Viral)
RN  - 0 (Capsid Proteins)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A Antigens)
RN  - 0 (VP6 protein, Rotavirus)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - Caco-2 Cells
MH  - Capsid Proteins/chemical synthesis/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - HLA-A Antigens/genetics/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Mice
MH  - Mice, Transgenic
MH  - Rotavirus/*immunology
MH  - Rotavirus Infections/immunology
MH  - Species Specificity
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2006/10/13 09:00
MHDA- 2006/11/15 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2006/11/15 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - 10.1099/vir.0.82031-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Nov;87(Pt 11):3393-3396. doi: 10.1099/vir.0.82031-0.