PMID- 17032748
OWN - NLM
STAT- MEDLINE
DCOM- 20070119
LR  - 20220129
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 37
IP  - 2
DP  - 2006 Oct
TI  - Expression profiling of 11beta-hydroxysteroid dehydrogenase type-1 and 
      glucocorticoid-target genes in subcutaneous and omental human preadipocytes.
PG  - 327-40
AB  - Obesity is associated with increased morbidity and mortality from cardiovascular 
      disease, diabetes and cancer. Although obesity is a multi-factorial heterogeneous 
      condition, fat accumulation in visceral depots is most highly associated with 
      these risks. Pathological glucocorticoid excess (i.e. in Cushing's syndrome) is a 
      recognised, reversible cause of visceral fat accumulation. The aim of this study 
      was to identify depot-specific glucocorticoid-target genes in adipocyte precursor 
      cells (preadipocytes) using Affymetrix microarray technique. Confluent 
      preadipocytes from subcutaneous (SC) and omental (OM) adipose tissue collected 
      from five female patients were treated for 24 h with 100 nM cortisol (F), RNA was 
      pooled and hybridised to the Affymetrix U133 microarray set. We identified 72 
      upregulated and 30 downregulated genes by F in SC cells. In OM preadipocytes, 56 
      genes were increased and 19 were decreased. Among the most interesting were 
      transcription factors, markers of adipocyte differentiation and glucose 
      metabolism, cell adhesion and growth arrest protein factors involved in G-coupled 
      and Wnt signalling. The Affymetrix data have been confirmed by quantitative 
      real-time PCR for ten specific genes, including HSD11B1, GR, C/EBPalpha, 
      C/EBPbeta, IL-6, FABP4, APOD, IRS2, AGTR1 and GHR. One of the most upregulated 
      genes in OM but not in SC cells was HSD11B1. The GR was similarly expressed and 
      not regulated by glucocorticoids in SC and OM human preadipocytes. C/EBPalpha was 
      expressed in SC preadipocytes and upregulated by F, but was below the detection 
      level in OM cells. C/EBPbeta was highly expressed both in SC and in OM 
      preadipocytes, but was not regulated by F. Our results provide insight into the 
      genes involved in the regulation of adipocyte differentiation by cortisol, 
      highlighting the depot specifically in human adipose tissue.
FAU - Bujalska, I J
AU  - Bujalska IJ
AD  - Division of Medical Sciences, The Medical School, Institute of Biomedical 
      Research, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
FAU - Quinkler, M
AU  - Quinkler M
FAU - Tomlinson, J W
AU  - Tomlinson JW
FAU - Montague, C T
AU  - Montague CT
FAU - Smith, D M
AU  - Smith DM
FAU - Stewart, P M
AU  - Stewart PM
LA  - eng
GR  - G0100729/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Glucocorticoids)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 1)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenase Type 1/*metabolism
MH  - Adipocytes/*metabolism
MH  - Adipose Tissue/metabolism
MH  - Adult
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Profiling/*methods
MH  - Glucocorticoids/*metabolism
MH  - Humans
MH  - Middle Aged
MH  - Omentum/*cytology/metabolism
MH  - Subcutaneous Fat/*metabolism
EDAT- 2006/10/13 09:00
MHDA- 2007/01/20 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/01/20 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - 37/2/327 [pii]
AID - 10.1677/jme.1.02048 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2006 Oct;37(2):327-40. doi: 10.1677/jme.1.02048.