PMID- 17032917
OWN - NLM
STAT- MEDLINE
DCOM- 20070306
LR  - 20220408
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 3
DP  - 2007 Feb 1
TI  - MCP-1 mediates TGF-beta-induced angiogenesis by stimulating vascular smooth 
      muscle cell migration.
PG  - 987-94
AB  - Transforming growth factor-beta (TGF-beta) and its signaling mediators play 
      crucial roles in vascular formation. Our previous microarray analysis identified 
      monocyte chemoattractant protein-1 (MCP-1) as a TGF-beta target gene in 
      endothelial cells (ECs). Here, we report that MCP-1 mediates the angiogenic 
      effect of TGF-beta by recruiting vascular smooth muscle cells (VSMCs) and 
      mesenchymal cells toward ECs. By using a chick chorioallantoic membrane assay, we 
      show that TGF-beta promotes the formation of new blood vessels and this promotion 
      is attenuated when MCP-1 activity is blocked by its neutralizing antibody. Wound 
      healing and transwell assays established that MCP-1 functions as a 
      chemoattractant to stimulate migration of VSMCs and mesenchymal 10T1/2 cells 
      toward ECs. Furthermore, the conditioned media from TGF-beta-treated ECs 
      stimulate VSMC migration, and inhibition of MCP-1 activity attenuates 
      TGF-beta-induced VSMC migration toward ECs. Finally, we found that MCP-1 is a 
      direct gene target of TGF-beta via Smad3/4. Taken together, our findings suggest 
      that MCP-1 mediates TGF-beta-stimulated angiogenesis by enhancing migration of 
      mural cells toward ECs and thus promoting the maturation of new blood vessels.
FAU - Ma, Jing
AU  - Ma J
AD  - State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of 
      Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
FAU - Wang, Qiang
AU  - Wang Q
FAU - Fei, Teng
AU  - Fei T
FAU - Han, Jing-Dong Jackie
AU  - Han JD
FAU - Chen, Ye-Guang
AU  - Chen YG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061010
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Cell Communication
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/*physiology
MH  - Chemotactic Factors
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology
MH  - Muscle, Smooth, Vascular/*cytology
MH  - *Neovascularization, Physiologic/drug effects
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2006/10/13 09:00
MHDA- 2007/03/07 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/03/07 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - S0006-4971(20)51982-4 [pii]
AID - 10.1182/blood-2006-07-036400 [doi]
PST - ppublish
SO  - Blood. 2007 Feb 1;109(3):987-94. doi: 10.1182/blood-2006-07-036400. Epub 2006 Oct 
      10.