PMID- 17033818
OWN - NLM
STAT- MEDLINE
DCOM- 20070326
LR  - 20181113
IS  - 0172-8083 (Print)
IS  - 0172-8083 (Linking)
VI  - 51
IP  - 1
DP  - 2007 Jan
TI  - Cytoplasmic Clb2 is required for timely inactivation of the mitotic inhibitor 
      Swe1 and normal bud morphogenesis in Saccharomyces cerevisiae.
PG  - 1-18
AB  - Subcellular localization is an important determinant of substrate and functional 
      specificity for cyclin-cyclin dependent kinase (CDK) complexes. This work 
      addresses the cytoplasmic function of the budding yeast mitotic cyclin Clb2, 
      which is mostly nuclear but is also present in the bulk cytoplasm and at the 
      mother-bud neck. Clb2 contains two leucine-rich nuclear export signals 
      (NESs)--one of which we newly describe here--that maintain its presence in the 
      cytoplasm. Yeast strains bearing mutations in one or both of these NESs have 
      elongated buds, indicative of a G2/M cell cycle delay. A small number of these 
      cells exhibit a filamentous-like morphology under conditions that do not normally 
      induce filamentous growth. These phenotypes are enhanced by deletion of the other 
      three mitotic cyclins (CLB1,3,4) and are dependent on expression of Swe1, the 
      yeast Cdk1 inhibitory kinase. Deltaclb1,3,4 Deltabud3 cells, which fail to 
      localize Clb2 to the bud neck, also exhibit a Swe1-dependent elongated bud 
      phenotype. Our results support a model in which cytoplasmic Clb2-Cdk1 is required 
      for timely inactivation of Swe1 at the G2/M transition and bud neck targeting of 
      Clb2 contributes to the efficiency of this process. Cytoplasmic Clb2 may also be 
      important for repression of filamentous growth.
FAU - Hood-DeGrenier, Jennifer K
AU  - Hood-DeGrenier JK
AD  - Department of Biological Sciences, Wellesley College, 106 Central St., Wellesley, 
      MA 02481, USA. jhooddeg@wellesley.edu
FAU - Boulton, Cortney N
AU  - Boulton CN
FAU - Lyo, Victoria
AU  - Lyo V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20061011
PL  - United States
TA  - Curr Genet
JT  - Current genetics
JID - 8004904
RN  - 0 (BUD3 protein, S cerevisiae)
RN  - 0 (CLB2 protein, S cerevisiae)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclins)
RN  - 0 (Nuclear Export Signals)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.1.- (SWE1 protein, S cerevisiae)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.22 (CDC28 Protein Kinase, S cerevisiae)
SB  - IM
MH  - Binding Sites
MH  - CDC28 Protein Kinase, S cerevisiae/metabolism
MH  - Cell Cycle Proteins/*antagonists & inhibitors
MH  - Cyclin B/analysis/genetics/*metabolism
MH  - Cyclins/genetics/metabolism
MH  - Cytoplasm/*metabolism
MH  - *Mitosis
MH  - Morphogenesis
MH  - Mutation
MH  - Nuclear Export Signals/genetics
MH  - Phenotype
MH  - Protein-Tyrosine Kinases/*antagonists & inhibitors
MH  - Saccharomyces cerevisiae/cytology/*growth & development/metabolism
MH  - Saccharomyces cerevisiae Proteins/analysis/*antagonists & 
      inhibitors/genetics/*metabolism
EDAT- 2006/10/13 09:00
MHDA- 2007/03/27 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/08/02 00:00 [received]
PHST- 2006/09/10 00:00 [accepted]
PHST- 2006/09/05 00:00 [revised]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/03/27 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - 10.1007/s00294-006-0102-1 [doi]
PST - ppublish
SO  - Curr Genet. 2007 Jan;51(1):1-18. doi: 10.1007/s00294-006-0102-1. Epub 2006 Oct 
      11.