PMID- 17033834
OWN - NLM
STAT- MEDLINE
DCOM- 20090514
LR  - 20181113
IS  - 0172-8172 (Print)
IS  - 0172-8172 (Linking)
VI  - 27
IP  - 1
DP  - 2006 Nov
TI  - Effect of dehydroepiandrosterone on cartilage and synovium of knee joints with 
      osteoarthritis in rabbits.
PG  - 79-85
AB  - The aim of this study was to investigate the effects of intra-articular injection 
      of dehydroepiandrosterone (DHEA) on cartilage and synovium of knee joints with 
      osteoarthritis (OA) in rabbits and the underlying mechanism. Forty rabbits 
      underwent unilateral anterior cruciate ligament transaction and were divided into 
      two groups. Rabbits were injected with 100 mumol/l DHEA dissolved in the 
      dimethylsulphoxide (DMSO) in the knee joints 5 weeks after transaction, once a 
      week for 5 weeks. Rabbits injected with DMSO under the same condition were served 
      as a control. All rabbits were killed 1 week after the last injection. The knee 
      joints were evaluated by gross morphology, histology, and gene expression 
      analysis. Gross morphologic inspection and histological evaluation showed that 
      the DHEA group appeared less damage in cartilage and synovium as compared with 
      the control. Gene expression analysis revealed that the mRNA expression of matrix 
      metalloproteinase-3 (MMP-3) in cartilage and synovium decreased significantly in 
      the DHEA group and that of tissue inhibitor of metalloproteinase-1 (TIMP-1) 
      increased. No significant difference of interleukin-1 beta (IL-1beta) mRNA 
      expression was found in the cartilage between two groups while the mRNA 
      expression of IL-1beta in the synovium was largely suppressed in the DHEA group. 
      The study suggests that DHEA plays a protective role against cartilage 
      degradation and synovium inflammation in rabbits with OA. This role may be 
      achieved through the regulation of the MMP-3, TIMP-1, and IL-1beta gene 
      expression in the cartilage and synovium.
FAU - Wu, Li Dong
AU  - Wu LD
AD  - Department of Orthopaedics, The 2nd Hospital of Medical College, Zhejiang 
      University, Hangzhou, People's Republic of China. ldwu@yahoo.com
FAU - Yu, Hua Chen
AU  - Yu HC
FAU - Xiong, Yan
AU  - Xiong Y
FAU - Feng, Jie
AU  - Feng J
LA  - eng
PT  - Journal Article
DEP - 20061011
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/surgery
MH  - Cartilage/*drug effects/metabolism/pathology
MH  - Dehydroepiandrosterone/administration & dosage/*pharmacology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Injections, Intra-Articular
MH  - Interleukin-1beta/metabolism
MH  - Knee Joint/*drug effects/metabolism/pathology
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Osteoarthritis/*metabolism/pathology
MH  - RNA, Messenger/metabolism
MH  - Rabbits
MH  - Synovial Membrane/*drug effects/metabolism/pathology
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
EDAT- 2006/10/13 09:00
MHDA- 2009/05/15 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/05/05 00:00 [received]
PHST- 2006/09/12 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2009/05/15 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - 10.1007/s00296-006-0238-9 [doi]
PST - ppublish
SO  - Rheumatol Int. 2006 Nov;27(1):79-85. doi: 10.1007/s00296-006-0238-9. Epub 2006 
      Oct 11.