PMID- 17034859
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20061218
IS  - 0147-9571 (Print)
IS  - 0147-9571 (Linking)
VI  - 30
IP  - 1
DP  - 2007 Jan
TI  - Porcine interleukin-2 gene encapsulated in chitosan nanoparticles enhances immune 
      response of mice to piglet paratyphoid vaccine.
PG  - 19-32
AB  - Interleukin-2 (IL-2) is vital to elicit and amplify the cellular and humoral 
      immune responses to foreign antigens, which is extensively utilized in the 
      control of infectious disease and treatment of various cancers. Porcine and 
      murine IL-2 genes were, respectively, subcloned into VR1020, designated as VPIL-2 
      and VMIL-2, and then encapsulated in chitosan nanoparticles (CNP) prepared by 
      ionic linkage. The BALB/c mice were intramuscularly co-administrated with 
      chitosan-IL-2 nanoparticles (CNP-IL2) and paratyphoid vaccine to test the 
      adjuvant effect of CNP-IL2. On day 35, the immunized mice were orally challenged 
      with virulent Salmonella. The content of IgG, IgA, IgM, IL-2, IL-4, IL-6 and 
      specific antibody titer as well as the number of immunocompetent cells were 
      systematically analyzed in the vaccinated mice. The results revealed that the 
      levels of immunoglobulins, cytokines, the specific antibodies, together with the 
      numbers of lymphocytes significantly increased in vaccinated mice inoculated with 
      CNP-VPIL2 in contrast with those with naked IL-2 plasmids and blank plasmids. The 
      CNP-VPIL2 immunized mice exhibited higher humoral and cellular immune responses, 
      less severe clinical signs and lesions of disease caused by the bacteria than the 
      other groups after challenge. These findings suggest that CNP-VPIL2 has a 
      significant enhancement effect on immune responses of mice, which results in 
      better immunoprotection against Salmonella infection, indicating that CNP-VPIL2 
      could be employed as an effective immunoadjuvant to elevate immunity of animals 
      to conventional vaccines.
FAU - Yang, Yi
AU  - Yang Y
AD  - Key Lab for Bio-Resource and Eco-Environment of Education Ministry, 
      Bioengineering Research Center for Animal Disease Prevention and Control, Life 
      Science College, Sichuan University, Wangjiang Road No. 29, Chengdu 610064, 
      China.
FAU - Chen, Jianlin
AU  - Chen J
FAU - Li, Hui
AU  - Li H
FAU - Wang, Yingyi
AU  - Wang Y
FAU - Xie, Zhao
AU  - Xie Z
FAU - Wu, Mei
AU  - Wu M
FAU - Zhang, Huan
AU  - Zhang H
FAU - Zhao, Zhongzhong
AU  - Zhao Z
FAU - Chen, Qian
AU  - Chen Q
FAU - Fu, Manliang
AU  - Fu M
FAU - Wu, Kaiyuan
AU  - Wu K
FAU - Chi, Cheng
AU  - Chi C
FAU - Wang, Hongning
AU  - Wang H
FAU - Gao, Rong
AU  - Gao R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061010
PL  - England
TA  - Comp Immunol Microbiol Infect Dis
JT  - Comparative immunology, microbiology and infectious diseases
JID - 7808924
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-2)
RN  - 0 (Typhoid-Paratyphoid Vaccines)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Adjuvants, Immunologic
MH  - Animals
MH  - Antibodies, Bacterial/*biosynthesis
MH  - Chitosan
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Immunoglobulins/*biosynthesis
MH  - Injections, Intramuscular
MH  - Interleukin-2/*immunology
MH  - Lymphocyte Activation/drug effects
MH  - Lymphocyte Count
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanoparticles
MH  - Random Allocation
MH  - Salmonella Infections, Animal/*immunology/prevention & control
MH  - Swine
MH  - Typhoid-Paratyphoid Vaccines/*immunology
EDAT- 2006/10/13 09:00
MHDA- 2007/02/21 09:00
CRDT- 2006/10/13 09:00
PHST- 2006/09/15 00:00 [accepted]
PHST- 2006/10/13 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/10/13 09:00 [entrez]
AID - S0147-9571(06)00061-0 [pii]
AID - 10.1016/j.cimid.2006.09.006 [doi]
PST - ppublish
SO  - Comp Immunol Microbiol Infect Dis. 2007 Jan;30(1):19-32. doi: 
      10.1016/j.cimid.2006.09.006. Epub 2006 Oct 10.